Therapeutic dendritic cell vaccine preparation using tumor RNA transfection: a promising approach for the treatment of prostate cancer.

Genetic Vaccines and Therapy
Juliana M Sousa-CanavezLuiz H Camara-Lopes

Abstract

Early prostate adenocarcinoma can be diagnosed through seric prostate-specific antigen (PSA) screenings. However, a fraction of patients progress to an incurable metastatic disease. Therefore, novel therapies for treating these patients are extremely desirable. Therapeutic vaccines based on Dendritic Cells (DCs) carrying tumor antigens have emerged as a promising strategy to initiate an immune response against tumor cells. These vaccines can be prepared using different methodologies, such as the application of tumor mRNA described in this work. Mature and immature DCs were obtained in vitro by adding specific cytokines to monocyte cell cultures. RNA extracted from prostate tumor lineage (LNCAP) was introduced into these cells by electroporation and co-incubation. Transfection success was measured by immunocytochemistry of the PSA expression level in DCs. Cell surface markers, including CD14, CD80, CD86, CCR7, CD11c, and CD1a, confirmed mature and immature DC phenotypes. Both cell maturation stages were successfully used for RNA introduction as shown by PSA characterization. Our data support the use of mature and immature DCs for vaccine preparation with either RNA electroporation or RNA co-incubation. The highest efficiency, ho...Continue Reading

References

Aug 1, 1996·The Journal of Experimental Medicine·D BoczkowskiE Gilboa
Apr 1, 1998·Nature·J Banchereau, R M Steinman
May 11, 1999·JAMA : the Journal of the American Medical Association·H I Scher
Jun 27, 2003·Cancer Immunology, Immunotherapy : CII·Dorian McIlroy, Marc Gregoire
Nov 5, 2003·American Journal of Human Biology : the Official Journal of the Human Biology Council·Sidia M Callegari-JacquesMara H Hutz
Jun 9, 2004·Cancer Immunology, Immunotherapy : CII·Jose Alexandre M BarbutoAntonio C Buzaid
Dec 16, 2006·International Reviews of Immunology·Takuya OsadaH Kim Lyerly
Apr 27, 2007·Current Urology Reports·Ased S M Ali, Freddie C Hamdy
May 4, 2007·The Journal of Clinical Investigation·Eli Gilboa
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Paul J MoscaH Kim Lyerly
May 22, 2007·International Journal of Cancer. Journal International Du Cancer·Anna-Katharina Thomas-KaskelHendrik Veelken
Sep 11, 2007·Urologic Oncology·Boris A Hadaschik, Martin E Gleave

❮ Previous
Next ❯

Citations

Oct 18, 2016·Cytotherapy·Rebecca S Abraham, Duane A Mitchell
Apr 24, 2019·Disability and Rehabilitation. Assistive Technology·Hana AlazemGenevieve Tibi
Oct 29, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Margit H LampenThorbald van Hall

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
density gradient centrifugation
flow cytometry
flow-cytometry
transfections
leukapheresis

Software Mentioned

Cell Quest Pro

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.