Therapeutic drug monitoring of carbamazepine and its metabolite in children from dried blood spots using liquid chromatography and tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Engy ShokryGiancarlo la Marca

Abstract

Carbamazepine (CBZ) is a first-line drug for the treatment of different forms of epilepsy and the first choice drug for trigeminal neuralgia. CBZ is metabolized in the liver by oxidation into carbamazepine-10,11-epoxide (CBZE), its major metabolite which is equipotent and known to contribute to the pharmacological activity of CBZ. The aim of the present study was to develop and validate a reliable, selective and sensitive liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of CBZ and its active metabolite in dried blood spots (DBS). The extraction process was carried out from DBS using methanol-water-formic acid (80:20:0.1, v/v/v). Chromatographic elution was achieved by using a linear gradient with a mobile phase consisting of acetonitrile-water-0.1% formic acid at a flow rate of 0.50mL/min. The method was linear over the range 1-40mg/L and 0.25-20mg/L for CBZ and CBZE, respectively. The limit of quantification was 0.75mg/L and 0.25mg/L for CBZ and CBZE. Intra-day and inter-day assay precisions were found to be lower than 5.13%, 6.46% and 11.76%, 4.72% with mean percentage accuracies of 102.1%, 97.5% and 99.2%, 97.8% for CBZ and CBZE. We successfully applied the method for determining DBS ...Continue Reading

References

Aug 1, 1992·The British Journal of Psychiatry : the Journal of Mental Science·G Stein
Jan 18, 1996·The New England Journal of Medicine·M J Brodie, M A Dichter
Dec 16, 1998·Therapeutic Drug Monitoring·J M Potter, A Donnelly
Sep 19, 2003·The New England Journal of Medicine·Gregory L KearnsRalph E Kauffman
Sep 24, 2004·Biomedical Chromatography : BMC·Miguel Navarrete CasasAlberto Fernández Gutiérrez
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Hugo M NeelsWilly E Lambert
Feb 14, 2006·Lancet·Renzo Guerrini
Apr 8, 2009·Therapeutic Drug Monitoring·Peter M EdelbroekLeo M L Stolk
Dec 18, 2009·Biomedical Chromatography : BMC·Wenkui Li, Francis L S Tse
May 27, 2010·Archives of Disease in Childhood·Parul PatelHitesh Pandya
Aug 17, 2010·Journal of Pharmaceutical and Biomedical Analysis·Giancarlo la MarcaRenzo Guerrini
Nov 23, 2010·British Journal of Clinical Pharmacology·Massimo CellaOscar Della Pasqua
Mar 12, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·N Mohamed ShahJ C McElnay
Oct 17, 2013·American Journal of Human Biology : the Official Journal of the Human Biology Council·Thomas W McDade

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Citations

May 18, 2016·Clinical Biochemistry·Marina Venzon AntunesRafael Linden
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Glenn LoosDeirdre Cabooter
Jan 29, 2017·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Lisa C MartialAlbert Batalla
Jan 6, 2018·Briefings in Bioinformatics·Rajeev K Azad, Vladimir Shulaev
Sep 8, 2018·Journal of Analytical Toxicology·Joana CarvalhoEugenia Gallardo
Jun 15, 2019·Basic & Clinical Pharmacology & Toxicology·Kyoung Lok MinMin Jung Chang
Mar 5, 2021·Biosensors & Bioelectronics·A HammoudM Sawan
Jun 6, 2021·Journal of Chromatography. a·Paweł Mateusz NowakRenata Wietecha-Posłuszny

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