Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries

Applied Microbiology and Biotechnology
Carina KrügerLennart Hammarström

Abstract

Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA revealed binding of the S36-VHH to the streptococcal antigen I/II adhesin molecule of S. mutans serotype C. In a rat-desalivated caries model, daily administration of S36-VHH significantly reduced the development of smooth surface caries. No additional therapeutic effect of GOx was observed. Our results suggest that llama VHH antibodies may be a potential benefit as prophylaxis against dental caries.

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Citations

Jun 17, 2009·Medical Microbiology and Immunology·Janusz WesolowskiFriedrich Koch-Nolte
Feb 7, 2009·Protein Engineering, Design & Selection : PEDS·Ana MonegalArio de Marco
Jan 25, 2011·Applied and Environmental Microbiology·M Cruz MartínHarold Marcotte
Sep 19, 2007·BMC Biotechnology·Anna HultbergHarold Marcotte
Jun 11, 2011·Microbial Cell Factories·Ario de Marco
Mar 19, 2013·Annual Review of Biochemistry·Serge Muyldermans
Aug 21, 2007·Applied Microbiology and Biotechnology·M M Harmsen, H J De Haard
Jul 31, 2012·Journal of Invertebrate Pathology·Guy CaljonJan Van Den Abbeele
Jul 1, 2010·Drug Discovery Today. Technologies
Apr 16, 2016·Drug Discovery Today·Sophie SteelandClaude Libert
Jan 20, 2011·Immunological Investigations·Fatemeh RahbarizadehZahra Sharifzadeh
Jul 20, 2019·Human Antibodies·Manzoor A MirSyed Suhail Hamdani

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