Therapeutic effects of adipose-tissue-derived mesenchymal stromal cells and their extracellular vesicles in experimental silicosis

Respiratory Research
Elga BandeiraMarcelo M Morales

Abstract

Silicosis is an occupational disease that affects workers who inhale silica particles, leading to extensive lung fibrosis and ultimately causing respiratory failure. Mesenchymal stromal cells (MSCs) have been shown to exert therapeutic effects in lung diseases and represent an alternative treatment for silicosis. Recently, it has been suggested that similar effects can be achieved by the therapeutic use of extracellular vesicles (EVs) obtained from MSCs. The aim of this study was to investigate the effects of adipose-tissue-derived MSCs (AD-MSCs) or their EVs in a model of silicosis. Silicosis was induced by intratracheal instillation of silica in C57BL/6 mice. After the onset of disease, animals received saline, AD-MSCs, or EVs, intratracheally. At day 30, AD-MSCs and EVs led to a reduction in collagen fiber content, size of granuloma, and in the number of macrophages inside granuloma and in the alveolar septa. In addition, the expression levels of interleukin 1β and transforming growth factor beta in the lungs were decreased. Higher dose of EVs also reduced lung static elastance when compared with the untreated silicosis group. Both AD-MSCs and EVs, locally delivered, ameliorated fibrosis and inflammation, but dose-enhanced E...Continue Reading

References

Mar 15, 2001·Journal of Applied Physiology·D S FaffeW A Zin
Jul 18, 2001·The Journal of Experimental Medicine·V M BorgesG A DosReis
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
May 11, 2006·Journal of Cell Science·Lindolfo da Silva MeirellesNance Beyer Nardi
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naveen GuptaMichael A Matthay
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Apr 9, 2008·Stem Cells and Development·Linyi PengChunyan Zhou
Sep 19, 2009·Respiratory Physiology & Neurobiology·Roberta M LassanceMarcelo M Morales
Mar 9, 2010·Respiratory Physiology & Neurobiology·Connie C W HsiaEwald R Weibel
Jun 19, 2010·American Journal of Respiratory and Critical Care Medicine·Shirley H J MeiDuncan J Stewart
Aug 10, 2010·The European Respiratory Journal·T Maron-GutierrezM M Morales
Aug 31, 2010·Translational Research : the Journal of Laboratory and Clinical Medicine·Viranuj Sueblinvong, Daniel J Weiss
Mar 23, 2011·Nature Biotechnology·Lydia Alvarez-ErvitiMatthew J A Wood
Jul 6, 2011·The Journal of Experimental Medicine·Thomas A Wynn
Apr 27, 2012·Lancet·Chi Chiu LeungWeihong Chen
Sep 11, 2012·Biotechnology Advances·Ruenn Chai LaiSai Kiang Lim
Oct 2, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lavinia IonescuBernard Thébaud
Oct 5, 2012·Stem Cells and Development·Stefania BrunoGiovanni Camussi
Nov 21, 2012·Respiratory Physiology & Neurobiology·Soraia C AbreuPatricia R M Rocco
Feb 20, 2013·Cancer Letters·Mark KatakowskiMichael Chopp
Apr 4, 2013·Respiratory Physiology & Neurobiology·Soraia C AbreuPatricia R M Rocco
Jul 23, 2013·Annals of the American Thoracic Society·Daniel J WeissDarwin Prockop
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Dec 21, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Miquéias Lopes-PachecoMarcelo M Morales
Feb 28, 2014·International Journal of Molecular Medicine·Cristina GrangeGiovanni Camussi
Aug 2, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luisa PascucciAugusto Pessina
Oct 3, 2014·Respiratory Research·Mariana A AntunesPatricia R M Rocco
Oct 16, 2014·Journal of Extracellular Vesicles·Jan Van DeunAn Hendrix
Apr 8, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S GennaiJ W Lee
Apr 23, 2015·Journal of Extracellular Vesicles·Oscar P B WiklanderSamir El Andaloussi
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever
Jun 13, 2015·American Journal of Respiratory and Critical Care Medicine·Antoine MonselJae W Lee
Jul 22, 2015·Inhalation Toxicology·Hajime Kawasaki
Aug 20, 2015·Stem Cells Translational Medicine·Oscar E SimonsonKatarina Le Blanc
Sep 2, 2015·Chest·Ltc Daniel E BanksSurinder K Jindal

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Citations

Oct 15, 2019·Expert Review of Respiratory Medicine·Fernanda Ferreira Cruz, Patricia Rieken Macedo Rocco
Apr 2, 2020·International Journal of Molecular Sciences·Erin N Worthington, James S Hagood
Oct 4, 2019·Scientific Reports·Jessica M MayeuxKenneth Michael Pollard
Nov 24, 2019·Seminars in Cell & Developmental Biology·Jason S RockelSowmya Viswanathan
Dec 22, 2020·Frontiers in Pharmacology·Linshen Xie, Ye Zeng
Apr 22, 2021·Chinese Medical Journal·Xian-Mei QiChen Wang
May 1, 2021·International Journal of Molecular Sciences·Jana Adamcakova, Daniela Mokra
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardAbbas Basiri

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Methods Mentioned

BETA
flow cytometry
ELISA
transmission electron microscopy
scanning electron microscopy
bronchoalveolar lavage

Software Mentioned

ImageJ
Image Pro Plus
Capture
GraphPad
GraphPad Prism

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