Therapeutic Effects of HIF-1α on Bone Formation around Implants in Diabetic Mice Using Cell-Penetrating DNA-Binding Protein

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Sang-Min OhJae-Hoon Lee

Abstract

Patients with uncontrolled diabetes are susceptible to implant failure due to impaired bone metabolism. Hypoxia-inducible factor 1α (HIF-1α), a transcription factor that is up-regulated in response to reduced oxygen during bone repair, is known to mediate angiogenesis and osteogenesis. However, its function is inhibited under hyperglycemic conditions in diabetic patients. This study thus evaluates the effects of exogenous HIF-1α on bone formation around implants by applying HIF-1α to diabetic mice and normal mice via a protein transduction domain (PTD)-mediated DNA delivery system. Implants were placed in the both femurs of diabetic and normal mice. HIF-1α and placebo gels were injected to implant sites of the right and left femurs, respectively. We found that bone-to-implant contact (BIC) and bone volume (BV) were significantly greater in the HIF-1α treated group than placebo in diabetic mice (p < 0.05). Bioinformatic analysis showed that diabetic mice had 216 differentially expressed genes (DEGs) and 21 target genes. Among the target genes, NOS2, GPNMB, CCL2, CCL5, CXCL16, and TRIM63 were found to be associated with bone formation. Based on these results, we conclude that local administration of HIF-1α via PTD may boost bone ...Continue Reading

References

Mar 15, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J R FunkS R Hurwitz
Jun 29, 2000·The Journal of Bone and Joint Surgery. British Volume·S A CorbettS P Hughes
Nov 25, 2004·Diabetes·Sergiu-Bogdan CatrinaLorenz Poellinger
Apr 29, 2005·Journal of Periodontology·Jeffrey A KopmanJoseph P Fiorellini
Apr 29, 2005·Journal of Periodontology·Peter T KwonJoseph P Fiorellini
Apr 17, 2007·Journal of Clinical Periodontology·Ghada AlsaadiDaniel van Steenberghe
Mar 4, 2008·Biochemical and Biophysical Research Communications·Oju JeonByung-Soo Kim
Dec 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ileana Ruxandra BotusanSergiu-Bogdan Catrina
Oct 16, 2009·Journal of Dental Research·B XuJ Chen
Jan 2, 2010·Biochemical and Biophysical Research Communications·Eun-Sung KimSang-Kyou Lee
Feb 9, 2010·Diabetes Research and Clinical Practice·Feng WangBao-gang Wang
Aug 17, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bing LiJeremy S Duffield
Dec 15, 2010·Journal of Periodontology·Zhao LinWilliam V Giannobile
Nov 25, 2011·Clinical Oral Implants Research·K A SchlegelC von Wilmowsky
Dec 14, 2011·Journal of Dental Research·P PapaspyridakosG O Gallucci
Jul 1, 2017·Molecular and Cellular Biochemistry·Mijeong JeonJe Seon Song

❮ Previous
Next ❯

Citations

Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin LiangQuan Zou

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
light microscopy

Software Mentioned

MATCH
gene
SPSS
ARRIVE
Tophat
TRANSFAC
Cuffdiff

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.