Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

BMC Complementary and Alternative Medicine
Mehdi KoushkiFatemeh Goshadrou

Abstract

Gastric ulcer is one of the most prevalent diseases worldwide. In Iranian folk medicine, Achillea wilhelmsii (AW) is used as a treatment for gastric ulcer. Previous reports also mentioned Antiulcerogenic properties for this herbal plant. This study investigated the therapeutic effects of Achillea wilhelmsii C. Koch extract on indomethacin-induced gastric lesion in rats, from both proteomic and metabolomic perspectives. The rats were divided into 4 groups. Gastric ulceration was induced by a single dose of indomethacin (45 mg/kg) by oral gavage. An amount of 800 mg/kg of AW extract was administered orally. Serum and tissue samples were collected for further investigations. The metabolomic study was performed by 1H-NMR CPMG spectrometry. Proteomic analysis was also executed by using two dimensional gel electrophoresis (2DE) followed by liquid chromatography coupled to tandem mass spectrometry (LC-ESI/MS/MS). Real time PCR was used to confirm some of the genes. The macroscopic and microscopic investigations confirmed the effectiveness of the AW extract. There was a panel of metabolites which showed alteration during gastric lesion development. The levels of some of these metabolite reversed nearly to their control values after the...Continue Reading

References

Sep 1, 1996·Progress in Lipid Research·J F Glatz, G J van der Vusse
Sep 16, 2000·Journal of Pain and Symptom Management·C Hawkins, G W Hanks
Feb 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Takeshi AiharaSusumu Okabe
Jul 5, 2005·Journal of Ethnopharmacology·Guillermo Schmeda-Hirschmann, Erdem Yesilada
May 2, 2006·Journal of Ethnopharmacology·Ana Maria CavalcantiMaria Consuelo Andrade Marques
Jun 6, 2007·The Journal of Physiological Sciences : JPS·V Nimet Izgüt-UysalPiraye Yargiçoğlu
Oct 4, 2007·Journal of Biochemical and Molecular Toxicology·Tarek K MotawiNancy N Shahin
May 1, 2008·Journal of Cancer Research and Clinical Oncology·Qiaojia HuangXu Lin
May 15, 2009·The Journal of Biological Chemistry·Shintaro SuemasuTohru Mizushima
Aug 17, 2010·Journal of Proteome Research·Eun-Kyung LeeChan-Wha Kim
Oct 12, 2010·Toxicology Letters·Jeeyoun JungGeum-Sook Hwang
Oct 19, 2010·Evidence-based Complementary and Alternative Medicine : ECAM·Biplab AdhikarySubrata Chattopadhyay
Mar 5, 2011·Journal of Clinical Biochemistry and Nutrition·Hirofumi MatsuiIchinosuke Hyodo
Jul 9, 2011·Biochemical Pharmacology·Tomoaki IshiharaTohru Mizushima
Mar 26, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yunyun ZhuWenjuan Cong
Apr 11, 2014·Mediators of Inflammation·Ana Flávia Teixeira RossiAna Elizabete Silva
Jun 1, 2014·Journal of Ethnopharmacology·L M R Al MuqarrabunS Ruzaina S Aris
Sep 28, 2014·Journal of Ethnopharmacology·Tahira BibiGhulam Yaseen
Nov 19, 2015·Acta Pharmaceutica Sinica. B·Aline Augusti BoligonLiliane de Freitas Bauermann
May 10, 2016·Nutrition·Nahla E El-AshmawyHend M Selim
Jun 9, 2016·The Journal of Biological Chemistry·Anthony E Pegg
Oct 25, 2016·Revista de gastroenterología de México·J A González-GonzálezH J Maldonado-Garza
Feb 10, 2017·Journal of Ethnopharmacology·Majid MohammadhosseiniAbolfazl Akbarzadeh
May 4, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Saleh Al-QuraishyAhmed Esmat Abdel Moneim
Nov 16, 2017·Nucleic Acids Research·David S WishartAugustin Scalbert

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Feb 29, 2020·International Journal of Molecular Sciences·Sachiko Koyama, Thomas Heinbockel
Sep 3, 2021·Planta medica·Thaise BoeingArquimedes Gasparotto Junior

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Methods Mentioned

BETA
electrophoresis
NMR
nuclear magnetic resonance
PCA
PCR

Software Mentioned

Mascot
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ProMetab
Visual Basic
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