Abstract
To observe the therapeutic effects of Saussurea involucrata (Sau) injection against severe acute pancreatitis (SAP)-induced brain injury. Sodium taurocholate-induced SAP-modeled rats were equally randomized into an SAP model group (SAP group) and a Sau treated group (Sau + S group). Healthy rats were equally randomized into a Sau treated group (Sau + H group) and a sham operation group (SO group). Serum amylase levels, endothelin-1 (ET-1) and nitric oxide (NO) contents were determined by optical turbidimetry, ELISA and nitrate reductase method respectively. Western blot was used to detect protein expression levels of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), ET-1, inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS) while mRNA levels of these biomarkers in brain tissue were measured by quantitative real-time PCR. Furthermore, pathological changes, as well as all above indexes of pancreas and brain, were observed at 6, 24 and 48 h after administration. There was a significant difference in mortality between SAP and Sau + S groups (P < 0.05). Serum amylase levels, ET-1 and NO contents, ET-1/NO ratio, relative expression levels of ET-1 and iNOS protein/mRNA of brain tissue in Sau + S group ...Continue Reading
References
Jan 1, 1992·Annals of Surgery·J SchmidtA L Warshaw
Apr 26, 2005·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Marko LempinenEsko Kemppainen
Aug 18, 2009·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Oliver MannTim Strate
Mar 23, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Anna Andrzejewska, Jan W Dlugosz
Jun 16, 2010·Nitric Oxide : Biology and Chemistry·Guy C Brown
Sep 16, 2010·Plant Cell Reports·Jian QiuFengshan Ma
Sep 17, 2010·Cell Biology International·Yabin WangFeng Cao
Nov 22, 2011·Peptides·Li WangDazhi Ke
Dec 15, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Ou JingminYe Qian
Oct 13, 2012·Frontiers in Neurology·Alejandro HornikJosé Biller
Dec 19, 2012·Journal of Pharmaceutical and Biomedical Analysis·Ridao ChenJungui Dai
Jan 8, 2013·Journal of the Neurological Sciences·E PaczkowskaB Machaliński
Apr 3, 2013·Cell Biochemistry and Biophysics·Huanchen ShaCui Liu
Oct 30, 2013·European Journal of Pain : EJP·E A CamargoS K P Costa
Dec 24, 2014·International Immunopharmacology·Xiaohong WangLing Mao
Jun 27, 2015·Journal of Ethnopharmacology·Wai-I ChikHu-Biao Chen
Dec 10, 2015·Pancreas·Lena TomkötterKai Bachmann
Citations
Oct 17, 2019·Digestive Diseases and Sciences·Xiaojia YangWeixing Wang
Feb 6, 2020·Frontiers in Pharmacology·Guowei GongTina Tx Dong
Oct 6, 2020·Journal of Analytical Methods in Chemistry·Qingdong MaJinhui Wang
Jul 13, 2021·Frontiers in Pharmacology·Qian ZhangXue Ying