Abstract
Although induced pluripotent stem (iPS) cell-derived cardiac constructs may have a potential in cardiomyogenesis of a distressed myocardium, obtaining polarity in cardiac constructs, such as via myocyte alignment, may be crucial to achieve a maximum contractile force for better clinical outcomes. We herein hypothesized that transplantation of an aligned cardiac tissue derived from iPS cells has therapeutic effects in a porcine ischemic cardiomyopathy model as a preclinical trial. Aligned cardiac tissues were developed by culturing high-purity iPS cell-derived cardiomyocytes in xeno-free conditions and transplanting them into infarct porcine hearts (iPS-CM group, n = 7; control, n = 6). Three months after treatment, therapeutic efficacy was evaluated functionally and histologically. In vitro assessment revealed that the aligned cardiac tissue containing high purity cardiomyocytes contracted homogeneously and had excellent mechanical properties. In the in vivo study, the left ventricular ejection fraction of the iPS-CM group was significantly greater than that of the control group, 3 months after transplantation (37.8% ± 2.3% vs 28.3% ± 2.5%, p < 0.05). Pathologically, attenuated interstitial fibrosis, attenuation of hypertrophie...Continue Reading
References
Mar 18, 2003·The Journal of Physiology·Sharon SiednerRobert Stehle
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaojun LianSean P Palecek
Sep 22, 2012·Circulation·Masashi KawamuraYoshiki Sawa
Oct 30, 2012·Cell Reports·Itsunari MinamiNorio Nakatsuji
Sep 18, 2013·Circulation·Masashi KawamuraYoshiki Sawa
Apr 30, 2014·Nature·James J H ChongCharles E Murry
Jun 16, 2014·Nature Methods·Paul W BurridgeJoseph C Wu
May 21, 2015·PloS One·Mahmood KhanMark G Angelos
Jan 1, 2016·The Journal of Biological Chemistry·Liying YanFuchou Tang
Oct 21, 2016·Nature·Yuji ShibaUichi Ikeda
Aug 20, 2017·Scientific Reports·Masashi KawamuraYoshiki Sawa
Sep 16, 2017·Circulation Research·Ali J Marian, Eugene Braunwald
Nov 7, 2017·Stem Cell Reports·Junjun LiLi Liu
Apr 9, 2018·The Journal of Thoracic and Cardiovascular Surgery·Shin YajimaYoshiki Sawa
Aug 18, 2018·Transplantation·Masaru IshidaYoshiki Sawa
Sep 16, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shohei YoshidaYoshiki Sawa
Jan 29, 2020·Bioactive Materials·Yuqian Jiang, Xiaojun Lance Lian
Mar 11, 2020·Journal of Bioscience and Bioengineering·Yumi SasanoMitsuru Akashi