Therapeutic normal IgG intravenous immunoglobulin activates Wnt-β-catenin pathway in dendritic cells.

Communications Biology
Anupama KarnamJagadeesh Bayry

Abstract

Therapeutic normal IgG intravenous immunoglobulin (IVIG) is a well-established first-line immunotherapy for many autoimmune and inflammatory diseases. Though several mechanisms have been proposed for the anti-inflammatory actions of IVIG, associated signaling pathways are not well studied. As β-catenin, the central component of the canonical Wnt pathway, plays an important role in imparting tolerogenic properties to dendritic cells (DCs) and in reducing inflammation, we explored whether IVIG induces the β-catenin pathway to exert anti-inflammatory effects. We show that IVIG in an IgG-sialylation independent manner activates β-catenin in human DCs along with upregulation of Wnt5a secretion. Mechanistically, β-catenin activation by IVIG requires intact IgG and LRP5/6 co-receptors, but FcγRIIA and Syk are not implicated. Despite induction of β-catenin, this pathway is dispensable for anti-inflammatory actions of IVIG in vitro and for mediating the protection against experimental autoimmune encephalomyelitis in vivo in mice, and reciprocal regulation of effector Th17/Th1 and regulatory T cells.

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Citations

Mar 26, 2021·International Immunopharmacology·Maryam BayatReyhaneh Eftekhari
Aug 24, 2021·Applied Microbiology and Biotechnology·Hilal Ahmad ParrayRajesh Kumar

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Methods Mentioned

BETA
nuclear translocation
ELISA
flow cytometry
electrophoresis
density gradient centrifugation
PCR
FCS
protein assay

Software Mentioned

myImageAnalysis
GolgiStop

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