Therapeutic polypeptides based on HBV core 18-27 epitope can induce CD8+ CTL-mediated cytotoxicity in HLA-A2+ human PBMCs

World Journal of Gastroenterology : WJG
Tong-Dong ShiWei Zhou

Abstract

To explore how to improve the immunogenicity of HBcAg CTL epitope based polypeptides and to trigger an HBV-specific HLA I-restricted CD8+ T cell response in vitro. A new panel of mimetic therapeutic peptides based on the immunodominant B cell epitope of HBV PreS2 18-24 region, the CTL epitope of HBcAg18-27 and the universal T helper epitope of tetanus toxoid (TT) 830-843 was designed using computerized molecular design method and synthesized by Merrifield's solid-phase peptide synthesis. Their immunological properties of stimulating activation and proliferation of lymphocytes, of inducing T( H1) polarization, CD8+ T cell magnification and HBV-specific CD8+ CTL mediated cytotoxicity were investigated in vitro using HLA-A2+ human peripheral blood mononuclear cells (PBMCs) from healthy donors and chronic hepatitis B patients. Results demonstrated that the therapeutic polypeptides based on immunodominant HBcAg18-27 CTL, PreS2 B- and universal T(H) epitopes could stimulate the activation and proliferation of lymphocytes, induce specifically and effectively CD8+ T cell expansion and vigorous HBV-specific CTL-mediated cytotoxicity in human PBMCs. It indicated that the introduction of immunodominant T helper plus B-epitopes with short ...Continue Reading

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Citations

Jan 25, 2017·Human Vaccines & Immunotherapeutics·Jianqiang LiJia Dou
May 30, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Murali GanesanNatalia A Osna
Jun 7, 2005·Expert Opinion on Drug Safety·Yutaka TsutsumiNobuo Masauzi
Nov 6, 2014·Jundishapur Journal of Microbiology·Azam AskariMohammad Kazemi Arababadi
Dec 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandra PhillipsNikolai V Naoumov
Nov 14, 2021·Alcoholism, Clinical and Experimental Research·Murali GanesanNatalia A Osna

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