Therapeutic potential of non-adherent BM-derived mesenchymal stem cells in tissue regeneration

Bone Marrow Transplantation
Z L ZhangD S Miao

Abstract

We demonstrated that non-adherent BM cells (NA-BMCs) can be expanded in suspension and give rise to multiple mesenchymal phenotypes including fibroblastic, osteoblastic, chondrocytic and adipocytic as well as glial cell lineages in vitro using the 'pour-off' BMC culture method. Mesenchymal stem cells (MSCs) derived from NA-BMCs (NA-MSCs) from wild-type mice were transplanted into VDR gene knockout (VDR(-/-)) mice that had received a lethal dose of radiation. Results revealed that NA-MSC can be used to rescue lethally irradiated mice and become incorporated into a diverse range of tissues. After lethal dose irradiation, all untransplanted mice died within 2 weeks, whereas those transplanted with NA-MSCs were viable for at least 3 months. Transplantation rescued these mice by reconstructing a hematopoietic system and repairing other damaged tissues. WBC, RBC and platelet counts recovered to normal after 1 month, and VDR gene expression was found in various tissues of viable VDR(-/-) recipients. Adult BM harbors pluripotent NA-MSCs, which can migrate in vivo into multiple body organs. In an appropriate microenvironment, they can adhere, proliferate and differentiate into specialized cells of target tissues and thus function in dam...Continue Reading

References

Sep 1, 1992·Bone and Mineral·A J FriedensteinI L Moskvina
May 1, 1991·European Journal of Haematology·E Clarke, S R McCann
Nov 1, 1990·The Journal of Clinical Investigation·M W LongK G Mann
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P AnklesariaJ S Greenberger
Mar 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A Scutt, P Bertram
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y C LiM B Demay
Apr 2, 1999·Science·M F PittengerD R Marshak
May 31, 2001·The Journal of Cell Biology·S A KuznetsovP G Robey
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·D K PandaD Goltzman
Feb 2, 2002·Experimental Hematology·Amelia BartholomewRonald Hoffman
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Dec 17, 2002·Arthritis and Rheumatism·Elena A JonesDennis McGonagle
Dec 31, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Henry E Young, Asa C Black
Mar 12, 2004·The Journal of Endocrinology·J Davies, T J Chambers
May 18, 2004·Experimental Hematology·Elisabeth H JavazonAlan W Flake
May 26, 2004·Transplant Immunology·Hairong Peng, Johnny Huard
Jun 10, 2004·Stem Cells and Development·Sonja LogesUrsula M Gehling
Oct 7, 2004·Current Opinion in Biotechnology·Bridget M DeasyJohnny Huard
Oct 20, 2004·Journal of Cellular and Molecular Medicine·D BakshR S Tuan
Nov 29, 2005·Ageing Research Reviews·Sebastian SetheAlexandra Stolzing
Jun 17, 2006·Stem Cells·Gaël Y RochefortJorge Domenech
Jul 4, 2006·Free Radical Biology & Medicine·Alexandra Stolzing, Andrew Scutt
Jan 18, 2007·The Journal of Experimental Medicine·Marta SerafiniCatherine M Verfaillie
May 15, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M KuciaM Z Ratajczak
Jun 15, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Seiichiro SakaoNorbert F Voelkel
Aug 8, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiao-Dong ChenRobert L Jilka

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Citations

May 8, 2010·Stem Cells and Development·Russell S TaichmanPaul H Krebsbach
Apr 21, 2011·Stem Cells and Development·Carmen L InsaustiJosé M Moraleda Jiménez
Apr 3, 2012·Stem Cells and Development·Alexandra StolzingAndrew Scutt
Oct 23, 2012·Stem Cell Research & Therapy·Kentaro AkiyamaSongtao Shi
Dec 31, 2014·Stem Cells International·Baldur Kristjánsson, Sittisak Honsawek
Jun 18, 2010·Cellular and Molecular Life Sciences : CMLS·Stephan FrickeUlrich Sack
Nov 20, 2012·Biotechnology Letters·Stefan PeterCatherine A Biggs
Aug 28, 2009·Histochemistry and Cell Biology·Elisa LeonardiDonatella Granchi
Sep 2, 2014·Advanced Drug Delivery Reviews·Martin J StoddartMauro Alini
Jan 15, 2013·Cytotherapy·Valeria RasiniRichard Schäfer
May 25, 2010·Immunology and Allergy Clinics of North America·Sung-Yun Pai, Luigi D Notarangelo
Mar 17, 2010·Best Practice & Research. Clinical Gastroenterology·Francesco Negro
Dec 24, 2011·International Journal of Biomaterials·A StolzingA Scutt
Oct 3, 2009·British Journal of Haematology·Vanderson RochaUNKNOWN Eurocord-Netcord registry and European Blood and Marrow Transplant group
Aug 6, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Heng-Wei ZhangDengshun Miao
Jul 18, 2015·Stem Cells International·Lindsay T McDonaldAmanda C LaRue
Apr 8, 2010·Clinical Lymphoma, Myeloma & Leukemia·Evelien KneppersSonja Zweegman
Sep 17, 2013·Pediatric Blood & Cancer·Gal GoldsteinAmos Toren
Sep 4, 2014·Journal of Dental Research·R C ZhengJ H Lee
Sep 30, 2016·World Journal of Transplantation·Darlene VigilAntonios H Tzamaloukas

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