Therapeutic Potential of Ocimum tenuiflorum as MPO Inhibitor with Implications for Atherosclerosis Prevention

Journal of Medicinal Food
Chandrakala Aluganti Narasimhulu, Sangamithra Vardhan

Abstract

Current experimental studies show that Ocimum tenuiflorum (commonly known as basil or Tulsi) possesses many health benefits. Ocimum is suggested to be antioxidative and anti-inflammatory. Eugenol, an orthomethoxyphenol, and ursolic acid have been identified as important components of basil. Myeloperoxidase (MPO), an oxidative enzyme, has been implicated in the pathogenesis of atherosclerosis. MPO-dependent oxidation of lipoproteins has been implicated in foam cell formation, dysfunctional HDL, and abnormalities in reverse cholesterol transport. Whole leaf extract of O. tenuiflorum and its major components, eugenol and ursolic acid, inhibit the oxidation of lipoproteins by myeloperoxidase/copper as measured by conjugated diene formation as well as by the thiobarbituric acid reactive substance (TBARS) assay. Whole basil leaf extract is able to attenuate the lipopolysaccharide-induced inflammation in RAW 264.7 cells compared with its components. In addition, whole basil leaf extract and eugenol inhibited MPO enzyme activity against synthetic substrates. Based on these results, we conclude that basil extract could act as an inhibitor of MPO and may serve as a nonpharmacological therapeutic agent for atherosclerosis.

References

Sep 1, 1979·Planta medica·H WagnerR Deininger
Jun 1, 1992·Oral Surgery, Oral Medicine, and Oral Pathology·K MarkowitzS Kim
Aug 1, 1992·International Journal of Food Microbiology·V Moleyar, P Narasimham
Dec 1, 1987·Neuropharmacology·J Feng, J M Lipton
Feb 1, 1972·Archives of Biochemistry and Biophysics·R K DesserE Shelton
Jul 15, 1983·Analytical Biochemistry·K SuzukiT Fujikura
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·U P SteinbrecherD Steinberg
Jun 1, 1995·The Journal of Clinical Investigation·N Santanam, S Parthasarathy
Jan 5, 2000·Journal of Ethnopharmacology·R R Chattopadhyay
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·R E Hancock, M G Scott
Sep 1, 2000·Journal of Ethnopharmacology·J Prakash, S K Gupta
Jul 9, 2002·Journal of Basic and Clinical Physiology and Pharmacology·Mukhtar AhmedM G Ghosesawar
Dec 20, 2002·Nature·Jonathan Cohen
Sep 16, 2004·Journal of Agricultural and Food Chemistry·Kohei KamiyaToshiko Satake
Sep 24, 2004·Physiological Reviews·Roland Stocker, John F Keaney
Sep 22, 2005·Clinical and Experimental Pharmacology & Physiology·Lien-Chai ChiangChun-Ching Lin
Oct 12, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·H RaghavenraK A Naidu
Oct 17, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·M Goretti V SilvaSelene M Morais
Sep 4, 2009·The Journal of Biological Chemistry·Arundhati UndurtiStanley L Hazen
Nov 11, 2010·The Journal of Biological Chemistry·Andrea GumieroPeter C E Moody
Mar 17, 2012·The Journal of Pharmacy and Pharmacology·Tatiana Fernanda BachiegaJosé Maurício Sforcin

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Citations

Nov 27, 2019·Future Medicinal Chemistry·Premkumar JayarajRajagopal Desikan
Aug 30, 2020·Pharmacological Reviews·Ana I CasasHarald H H W Schmidt

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Methods Mentioned

BETA
protein assay
electrophoresis
PCR

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