Therapeutic Potential of Sclareol in Experimental Models of Rheumatoid Arthritis

International Journal of Molecular Sciences
Sen-Wei TsaiChi-Chien Lin

Abstract

Previous studies have shown that the natural diterpene compound, sclareol, potentially inhibits inflammation, but it has not yet been determined whether sclareol can alleviate inflammation associated with rheumatoid arthritis (RA). Here, we utilized human synovial cell line, SW982, and an experimental murine model of rheumatoid arthritis, collagen-induced arthritis (CIA), to evaluate the therapeutic effects of sclareol in RA. Arthritic DBA/1J mice were dosed with 5 and 10 mg/kg sclareol intraperitoneally every other day over 21 days. Arthritic severity was evaluated by levels of anti-collagen II (anti-CII) antibody, inflammatory cytokines, and histopathologic examination of knee joint tissues. Our results reveal that the serum anti-CII antibody, cytokines interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and IL-17, as well as Th17 and Th1 cell population in inguinal lymph nodes, were significantly lower in sclareol-treated mice compared to the control group. Also, the sclareol treatment groups showed reduced swelling in the paws and lower histological arthritic scores, indicating that sclareol potentially mitigates collagen-induced arthritis. Furthermore, IL-1β-stimulated SW982 cells secreted less inflam...Continue Reading

References

Mar 20, 1999·Planta medica·M SinghR P Sharma
Mar 25, 2004·In Vitro Cellular & Developmental Biology. Animal·Taisuke YamazakiTakayoshi Tokiwa
May 26, 2007·Nature Reviews. Immunology·Iain B McInnes, Georg Schett
Oct 31, 2007·Autoimmunity Reviews·Young-Gyu ChoHo-Youn Kim
Mar 3, 2010·Immunological Reviews·Beatrix Bartok, Gary S Firestein
Mar 3, 2010·Immunological Reviews·Sook Kyung ChangMichael B Brenner
Jun 6, 2014·Journal of Immunology Research·Kohsuke YoshidaAkira Hashiramoto
Jul 8, 2014·Joint, Bone, Spine : Revue Du Rhumatisme·Alia FazaaLeith Zakraoui
Sep 13, 2015·The American Journal of Pathology·Kyoung-Woon KimSang-Heon Lee
Nov 26, 2015·Journal of Biomolecular Screening·Felicity D HerringtonCarl S Goodyear
Dec 15, 2015·International Immunopharmacology·Lin ZhangXiaofeng Li
Apr 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan-Jun SunTao Liu
Jan 14, 2017·Cell·Qian ZhangDavid Baltimore
Jun 2, 2017·Arthritis Research & Therapy·Marta F BustamanteMonica Guma
Jul 29, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Quy T N TranChristina L L Chai

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Citations

Apr 17, 2019·International Journal of Molecular Sciences·Chih-Hsin Tang
Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Venelina PopovaValtcho D Zheljazkov
Apr 8, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Hua CuiXiaojuan He
Jan 7, 2021·Environmental Science and Pollution Research International·Mona Awad AlonaziAbir Ben Bacha
May 3, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zheng XiangChaojie Wang
Jul 2, 2021·Journal of Ethnopharmacology·Yuan WangYan-Xu Chang

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
flow cytometry
ELISA
nuclear translocation
PMA
Protein Assay
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad
ImageJ

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