PMID: 16629382Apr 25, 2006Paper

Therapeutic potential of siRNA-mediated transcriptional gene silencing

BioTechniques
Kevin V Morris

Abstract

RNA interference (RNAi) and specifically the use of small interfering RNAs (siRNAs) represents a potentially new paradigm in gene knockout technology. Clearly siRNAs can be used to knockdown the expression of a targeted transcript in what has been termed posttranscriptional gene silencing (PTGS). While there are a plethora of reports applying siRNA-mediated PTGS the limitation of the duration of the effect remains. Recently, in human cells, siRNAs have been shown, similar to plants and Schizosaccharomyces pombe, to mediate transcriptional gene silencing (TGS). The observation that siRNAs can function in a TGS manner in human cells suggests that, similar to plants, human genes may also be able to be silenced more permanently via epigenetic modifications. The ramifications of siRNA-mediated TGS in humans suggest that longer term suppression of gene function can be obtained via siRNA-directed chromatin modifications. Undoubtedly the potential to employ siRNA technology is broader than once envisioned in human cells and suggests that siRNA-mediated TGS is not simply limited to PTGS. The potential to utilize siRNAs to direct epigenetic changes in local chromatin structure offers a new therapeutic avenue that could prove remarkably r...Continue Reading

References

Feb 11, 1994·Cell·M WasseneggerH L Sänger
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·B DropulićP M Pitha
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K MontgomeryA Fire
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·B D StrahlC D Allis
Dec 30, 1999·Nature Genetics·F FuksT Kouzarides
Aug 17, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B M Turner
Mar 10, 2001·Genes & Development·P A Sharp
Aug 11, 2001·Science·T Jenuwein, C D Allis
Mar 15, 2002·Current Opinion in Genetics & Development·Shelley L Berger
Aug 17, 2002·Current Opinion in Plant Biology·Alexandra Lusser
Aug 24, 2002·Science·Thomas A VolpeRobert A Martienssen
Aug 29, 2002·The EMBO Journal·Andrew HamiltonDavid Baulcombe
Sep 7, 2002·Science·Ira M HallShiv I S Grewal
Nov 14, 2002·Annual Review of Genetics·Marcel TijstermanRonald H A Plasterk
Apr 25, 2003·Nucleic Acids Research·François FuksTony Kouzarides
Jan 6, 2004·Science·André VerdelDanesh Moazed
Apr 14, 2004·Current HIV Research·Kevin V Morris, John J Rossi
Jul 23, 2004·Nature·Zachary LippmanRob Martienssen
Aug 7, 2004·Science·Kevin V MorrisDavid J Looney
Sep 8, 2004·Biochemical and Biophysical Research Communications·Chang Won ParkClifford J Steer
Jan 12, 2005·Nature Structural & Molecular Biology·G Brett RobbTariq M Rana
Feb 1, 2005·FEBS Letters·Hinrich BoegerRoger D Kornberg
Feb 22, 2005·The Journal of Biological Chemistry·Marc-André LangloisNan Sook Lee
May 4, 2005·Molecular Cell·Marc BühlerOliver Mühlemann
Jun 21, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Daniela CastanottoJohn J Rossi
Sep 6, 2005·Science·S KatayamaUNKNOWN FANTOM Consortium
Jan 5, 2006·Human Gene Therapy·Kevin V Morris, David J Looney
Jan 13, 2006·Nature Chemical Biology·Bethany A JanowskiDavid R Corey

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Citations

Dec 11, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Anne-Marie W TurnerKevin V Morris
Jun 6, 2008·Journal de la Société de biologie·Sophie BarralAndré Verdel
Dec 8, 2006·The New England Journal of Medicine·René Bernards
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·M MatzkeA J M Matzke
Nov 24, 2007·Expert Opinion on Biological Therapy·Kenneth A Howard, Jørgen Kjems
Dec 23, 2016·Nucleic Acids Research·Agathe EckenfelderSarah Gallois-Montbrun
Aug 23, 2018·Journal of Cellular Physiology·Habib ZarredarSafar Farajnia
Apr 25, 2007·NMR in Biomedicine·Venu RamanZaver M Bhujwalla
Mar 6, 2008·The Journal of Clinical Endocrinology and Metabolism·John J PelusoKathryn Phoenix
Mar 15, 2012·Cellular and Molecular Life Sciences : CMLS·Bing-Xue Yan, Jin-Xia Ma

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