Therapeutic Potential of Vitamin D and Curcumin in an In Vitro Model of Alzheimer Disease

Journal of Central Nervous System Disease
Abir Abdullah AlamroSamina Hyder Haq

Abstract

Alzheimer disease is a progressive neurodegenerative disease, affecting a very high proportion of the aging population. Several studies have demonstrated that one of the main contributors to this disease is oxidative stress (OS), which causes peroxidation of protein, lipids, and DNA resulting in the formation of advanced glycosylated end products (AGE) in the brain tissues. These AGE are usually associated with the amyloid β (Aβ), which could further aggravate its toxicity and its clearance. Antioxidants counteract the deterioration caused by OS. We aimed to evaluate the effect of vitamin D3 and curcumin on primary cortical neuronal cultures exposed to Aβ1-42 toxicity for different time periods. Primary cortical neuronal cultures were set up and exposed to Aβ1-42 for up to 72 hours. Cell viability was studied by 3[4,5-dimethylthiazole-2-yl]-2,5-dipheyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay. Biochemical assays for OS such as lipid peroxidation, reduced Glutathione(GSH), Glutathione S-transferase (GST), catalase, and superoxide dismutase (SOD) were conducted. Sandwich enzyme-linked immunosorbent assay (ELISA) was used to study the neurotrophic growth factor (NGF) expression. Treatments with Aβ1-42 caused ...Continue Reading

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Citations

Sep 26, 2020·Brain Sciences·Mahitab FarghaliFrancesca Uberti
Jan 8, 2021·Expert Review of Neurotherapeutics·Francesco PanzaMadia Lozupone
Dec 31, 2020·International Journal of Molecular Sciences·Umair ShabbirDeog-Hwan Oh

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
Assay
MDA

Software Mentioned

Prism

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