Therapeutic ratio quantifies laser antisepsis: ablation of Porphyromonas gingivalis with dental lasers

Lasers in Surgery and Medicine
David M Harris, Michael Yessik

Abstract

It is established that both pulsed Nd:YAG (1,064 nm) and continuous diode (810 nm) dental lasers kill pathogenic bacteria (laser antisepsis), but a quantitative method for determining clinical dosimetry does not exist. The purpose of this study was to develop a method to quantify the efficacy of ablation of Porphyromonas gingivalis (Pg) in vitro for two different lasers. The ablation thresholds for the two lasers were compared in the following manner. The energy density was measured as a function of distance from the output of the fiber-optic delivery system. Pg cultures were grown on blood agar plates under standard anaerobic conditions. Blood agar provides an approximation of gingival tissue for the wavelengths tested in having hemoglobin as a primary absorber. Single pulses of laser energy were delivered to Pg colonies and the energy density was increased until the appearance of a small plume was observed coincident with a laser pulse. The energy density at this point defines the ablation threshold. Ablation thresholds to a single pulse were determined for both Pg and for blood agar alone. The large difference in ablation thresholds between the pigmented pathogen and the host matrix for pulsed-Nd:YAG represented a significan...Continue Reading

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Citations

May 25, 2005·Photomedicine and Laser Surgery
Jun 16, 2005·Photomedicine and Laser Surgery
Sep 8, 2005·Photomedicine and Laser Surgery
Apr 6, 2006·Journal of Periodontology·Charles M Cobb
Jun 16, 2014·The Journal of Evidence-based Dental Practice·Samuel B Low, Angie Mott
Mar 27, 2007·British Dental Journal·S Parker
Apr 6, 2016·Periodontology 2000·Koji MizutaniYuichi Izumi
Jun 23, 2016·Journal of Lasers in Medical Sciences·Mohammadreza TalebiMaryam Torshabi
Aug 2, 2016·Lasers in Surgery and Medicine·David M HarrisRichard Darveau
Oct 19, 2016·Lasers in Surgery and Medicine·David M Harris, Lou Reinisch
Aug 2, 2017·Periodontology 2000·Charles M Cobb
Jul 17, 2009·Journal of the American Podiatric Medical Association·Eric Bornstein
Aug 30, 2020·International Journal of Environmental Research and Public Health·Elisabetta PolizziGiorgio Gastaldi
Jul 22, 2018·Journal of Lasers in Medical Sciences·Amrita JhaRoopa Adinarayan
Sep 27, 2019·International Journal of Molecular Sciences·Marco GiannelliDaniele Bani
Oct 31, 2020·Photobiomodulation, Photomedicine, and Laser Surgery·Kinga Grzech-LeśniakJanina Golob Deeb

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