Thermal activation of single kinesin molecules with temperature pulse microscopy

Cell Motility and the Cytoskeleton
K Kawaguchi, S Ishiwata

Abstract

Conventional kinesin is a processive motor protein that keeps "walking" along a microtubule using chemical energy released by ATP hydrolysis. We previously studied the effects of temperature between 15 degrees and 35 degrees C on the moving velocity, force, and processivity of single kinesin molecules using a bead assay [Kawaguchi and Ishiwata, 2000b: Biochem Biophys Res Commun 272:895-899]. However, we could not examine the effects of temperature higher than 35 degrees C because of the thermal damage to proteins. Here, using temperature pulse microscopy (TPM) [Kato et al., 1999: Proc Natl Acad Sci USA 96:9602-9606], we could examine the temperature dependence of the gliding velocity of single kinesin molecules interacting with a microtubule above 35 degrees C up to 50 degrees C (instantaneously, approximately 60 degrees C), where the velocity reached 3.68 microm/s, the highest ever reported. The Arrhenius plot showed no breaks between 15 degrees and 50 degrees C with a unique activation energy of about 50 kJ/mol, suggesting that the molecular mechanism of kinesin motility is common over a broad temperature range including physiological temperature.

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Citations

May 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nicholas R Guydosh, Steven M Block
Jan 13, 2006·Nature Chemical Biology·Yuichi TaniguchiToshio Yanagida
Feb 27, 2007·Bio Systems·Yuichi TaniguchiToshio Yanagida
May 31, 2008·Biotechnology and Bioengineering·Adrienne C GreeneGeorge D Bachand
Jan 30, 2007·Biophysical Journal·Julien S Davis, Neal D Epstein
Nov 17, 2015·Scientific Reports·Kotaro OyamaShin'ichi Ishiwata
Feb 13, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Andrew E PellingJames K Gimzewski
Jul 23, 2015·Biophysical Journal·Kotaro OyamaShin'ichi Ishiwata
Dec 11, 2007·Biochemical and Biophysical Research Communications·Kenji KawaguchiToshihide Yamashita
Jul 18, 2006·Biophysical Journal·Bradley C SteelCristobal G dos Remedios
Sep 23, 2010·Biophysical Journal·Liming WangGerman Enciso
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Hideyuki F Arata, Hiroyuki Fujita
Jan 20, 2019·Biotechnology and Bioengineering·Feiran LiJong Hyun Choi
Apr 24, 2019·The Journal of General Physiology·Shuya IshiiShin'ichi Ishiwata
Mar 1, 2016·Biophysics Reviews·Yuichi Inoue, Akihiko Ishijima
Feb 22, 2006·Biophysics·Ikuko Nara, Shin'ichi Ishiwata
Oct 8, 2017·Biochemical and Biophysical Research Communications·K ChaseM Vershinin
Mar 19, 2021·Frontiers in Cell and Developmental Biology·Saumya Yadav, Ambarish Kunwar
Aug 25, 2004·Science·Andrew E PellingJames K Gimzewski

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