Thermal adaptation strategies of the extremophile bacterium Thermus filiformis based on multi-omics analysis

Extremophiles : Life Under Extreme Conditions
Fernanda MandelliFabio Marcio Squina

Abstract

Thermus filiformis is an aerobic thermophilic bacterium isolated from a hot spring in New Zealand. The experimental study of the mechanisms of thermal adaptation is important to unveil response strategies of the microorganism to stress. In this study, the main pathways involved on T. filiformis thermoadaptation, as well as, thermozymes with potential biotechnological applications were revealed based on omics approaches. The strategy adopted in this study disclosed that pathways related to the carbohydrate metabolism were affected in response to thermoadaptation. High temperatures triggered oxidative stress, leading to repression of genes involved in glycolysis and the tricarboxylic acid cycle. During heat stress, the glucose metabolism occurred predominantly via the pentose phosphate pathway instead of the glycolysis pathway. Other processes, such as protein degradation, stringent response, and duplication of aminoacyl-tRNA synthetases, were also related to T. filiformis thermoadaptation. The heat-shock response influenced the carotenoid profile of T. filiformis, favoring the synthesis of thermozeaxanthins and thermobiszeaxanthins, which are related to membrane stabilization at high temperatures. Furthermore, antioxidant enzyme...Continue Reading

References

Dec 1, 1991·Journal of Bacteriology·F LévêqueP Plateau
May 1, 1967·The Biochemical Journal·N S Willetts
May 1, 1967·The Biochemical Journal·N S Willetts
Apr 1, 1981·Journal of Bacteriology·I N HirshfieldF C Neidhardt
Jun 13, 1998·The Journal of Biological Chemistry·T D CaldasG Richarme
Jul 24, 1998·Extremophiles : Life Under Extreme Conditions·M C EgasE M Pires
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·H D Becker, D Kern
Nov 11, 1999·Biochimica Et Biophysica Acta·M HaraJ Miyake
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Apr 6, 2002·Applied Microbiology and Biotechnology·J H MartensD Jahn
Jun 18, 2003·International Journal of Food Microbiology·Kaarel AdambergToomas Paalme
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jan 17, 2004·Applied Microbiology and Biotechnology·F BlascoR D Schmid
Oct 6, 2004·Genome Biology·Donal A Hickey, Gregory A C Singer
Nov 2, 2004·Journal of Bacteriology·Haichun GaoJizhong Zhou
Jan 5, 2005·Applied Microbiology and Biotechnology·T Panda, B S Gowrishankar
Jul 7, 2005·Extremophiles : Life Under Extreme Conditions·Kentaro Miyazaki
Dec 7, 2006·Journal of Agricultural and Food Chemistry·Antonio FiorentinoPietro Monaco
Aug 2, 2007·PloS One·Ryan J MaillouxVasu D Appanna
Dec 25, 2007·Journal of Biology·Markus RalserSylvia Krobitsch
Dec 18, 2009·BMC Bioinformatics·Alvaro Cuadros-InostrozaMatthew A Hannah
May 13, 2010·Molecular Systems Biology·Szymon JozefczukLothar Willmitzer
Jan 11, 2011·Bioinformatics·Guillaume Marçais, Carl Kingsford
Feb 18, 2012·Nature Reviews. Microbiology·Zachary D Dalebroux, Michele S Swanson
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Fernanda MandelliAdriana Z Mercadante
Oct 17, 2012·Bioinformatics·Evguenia KopylovaHélène Touzet
Mar 28, 2013·Letters in Applied Microbiology·F MandelliF M Squina
Apr 6, 2013·Nucleic Acids Research·Yang LiaoWei Shi

❮ Previous
Next ❯

Citations

Nov 6, 2018·Folia Microbiologica·Bhagwan Rekadwad, Juan M Gonzalez
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Daochen ZhuJianzhong Sun
Sep 22, 2019·Journal of Hazardous Materials·Patricia GiovanellaLara D Sette
Oct 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Yuanyuan PanDa-Wen Sun

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRR5025492
JPSL02000000

Methods Mentioned

BETA
RNA-seq
glycosylation
PCR
in vitro transcription

Software Mentioned

R
R Development Core Team
EdgeR
Rsubread package
Target Search R - package
gplots
Mascot
Mascot Distiller
Sequest
Leco ChromaTOF

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.