Thermal Bioprinting Causes Ample Alterations of Expression of LUCAT1, IL6, CCL26, and NRN1L Genes and Massive Phosphorylation of Critical Oncogenic Drug Resistance Pathways in Breast Cancer Cells

Frontiers in Bioengineering and Biotechnology
Aleli CampbellThomas Boland

Abstract

Bioprinting technology merges engineering and biological fields and together, they possess a great translational potential, which can tremendously impact the future of regenerative medicine and drug discovery. However, the molecular effects elicited by thermal inkjet bioprinting in breast cancer cells remains elusive. Previous studies have suggested that bioprinting can be used to model tissues for drug discovery and pharmacology. We report viability, apoptosis, phosphorylation, and RNA sequence analysis of bioprinted MCF7 breast cancer cells at separate timepoints post-bioprinting. An Annexin A5-FITC apoptosis stain was used in combination with flow cytometry at 2 and 24 h post-bioprinting. Antibody arrays using a Human phospho-MAPK array kit was performed 24 h post-bioprinting. RNA sequence analysis was conducted in samples collected at 2, 7, and 24 h post-bioprinting. The post-bioprinting cell viability averages were 77 and 76% at 24 h and 48 h, with 31 and 64% apoptotic cells at 2 and 24 h after bioprinting. A total of 21 kinases were phosphorylated in the bioprinted cells and 9 were phosphorylated in the manually seeded controls. The RNA seq analysis in the bioprinted cells identified a total of 12,235 genes, of which 9.7%...Continue Reading

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Citations

Jan 26, 2021·Translational Oncology·Robin AugustineAnwarul Hasan
Jun 23, 2021·Essays in Biochemistry·Byeongwook JoShoji Takeuchi
Jul 15, 2020·Chemical Reviews·Mihyun LeeMarcy Zenobi-Wong
Sep 17, 2020·Chemical Reviews·Ana Clotilde FonsecaMarco Domingos
Oct 7, 2021·Advanced Materials·Riccardo RizzoMarcy Zenobi-Wong

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Methods Mentioned

BETA
bioprinting
Antibody
Profiler
RNA seq
bioprinter

Software Mentioned

[UNK] Analysis
SPSS Statistics
Cufflinks
Bowtie2
[UNK] [UNK] Analysis
Panther
Minitab
Kaluza
Zen
STRING

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