Thermal broadening analysis of the light harvesting complex II absorption spectrum

Biochemistry
Giuseppe ZucchelliRobert C Jennings

Abstract

Absorption spectra in the Qy region of the light harvesting complex II (LHCII) have been measured in the temperature range 70-300 K. The spectra were analyzed by evaluating the temperature dependence (a) of the total bandwidth and (b) of the sub-bands obtained by numerical decomposition in terms of double Gaussians. The thermal broadening of the bands are interpreted, in both cases, as a homogeneous component, due to the presence of linear electron-phonon coupling, plus an inhomogeneous component, due to both statistical energy fluctuations at each pigment site and heterogeneity of the sample itself. Sub-bands analysis, in which eight major components are identified, yields a reorganization energy 9 cm(-1) < or = Svm < or = 14 cm(-1) and an inhomogeneous contribution in the range 120-170 cm(-1). In all cases the bands are substantially symmetrical in the 70-300 K temperature range. This observation gains theoretical support from an analysis of the band moments, when the influence of a low-frequency vibrational mode is considered. Analysis of the total absorption band yields Svm approximately 70 cm(-1); however, this high value is reduced to Svm approximately 11-20 cm(-1) when the red-most sub-band, with maximum at 684 nm, is el...Continue Reading

Citations

Jun 7, 2006·Photosynthesis Research·Giorgio FortiGiuseppe Zucchelli
Apr 7, 2005·FEBS Letters·Giuseppe ZucchelliRobert C Jennings
Feb 28, 2001·FEBS Letters·S SantabarbaraR C Jennings
Dec 8, 2014·Biochimica Et Biophysica Acta·Stefano SantabarbaraDonatella Carbonera
Aug 25, 2015·Journal of Photochemistry and Photobiology. B, Biology·Alonso ZavaferMun Hon Cheah
May 17, 2005·Journal of Biochemical and Biophysical Methods·Igor K MikhailyukAndrej P Razjivin
Jul 9, 2004·Biophysical Journal·Robert C JenningsFlavio M Garlaschi
Dec 26, 2001·Biophysical Journal·Giuseppe ZucchelliOliviero Cremonesi
Aug 9, 2003·Biochimica Et Biophysica Acta·Miguel A PalaciosHerbert van Amerongen
Jan 25, 2002·Biophysical Journal·Axel SchubertHeiko Lokstein
Feb 11, 2015·The Journal of Physical Chemistry. B·Kamarniso VrandecicJörg Pieper
Jul 17, 2019·The Journal of Physical Chemistry. B·Parveen AkhtarHowe-Siang Tan

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