Thermal characterization and analysis of microliter liquid volumes using the three-omega method

The Review of Scientific Instruments
Shilpi Roy-PanzerKenneth E Goodson

Abstract

Thermal phenomena in many biological systems offer an alternative detection opportunity for quantifying relevant sample properties. While there is substantial prior work on thermal characterization methods for fluids, the push in the biology and biomedical research communities towards analysis of reduced sample volumes drives a need to extend and scale these techniques to these volumes of interest, which can be below 100 pl. This work applies the 3ω technique to measure the temperature-dependent thermal conductivity and heat capacity of de-ionized water, silicone oil, and salt buffer solution droplets from 24 to 80 °C. Heater geometries range in length from 200 to 700 μm and in width from 2 to 5 μm to accommodate the size restrictions imposed by small volume droplets. We use these devices to measure droplet volumes of 2 μl and demonstrate the potential to extend this technique down to pl droplet volumes based on an analysis of the thermally probed volume. Sensitivity and uncertainty analyses provide guidance for relevant design variables for characterizing properties of interest by investigating the tradeoffs between measurement frequency regime, device geometry, and substrate material. Experimental results show that we can ext...Continue Reading

References

Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·T V ChalikianK J Breslauer
Sep 4, 2007·The Review of Scientific Instruments·Sun Rock ChoiDongsik Kim
Jul 8, 2008·The Review of Scientific Instruments·Sun Rock Choi, Dongsik Kim
Mar 5, 2009·The Review of Scientific Instruments·Seung-Min Lee
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wonhee LeeMichael L Roukes
Jun 10, 2011·Lab on a Chip·Namwoo YiJaesung Park
Jul 5, 2011·The Review of Scientific Instruments·Scott N Schiffres, Jonathan A Malen
Nov 7, 2012·The Review of Scientific Instruments·Byoung Kyoo ParkDongsik Kim
Jul 6, 2014·The Review of Scientific Instruments·Matthew L Bauer, Pamela M Norris

❮ Previous
Next ❯

Citations

Aug 19, 2015·ACS Applied Materials & Interfaces·Michael T BarakoKenneth E Goodson
Apr 7, 2021·The Review of Scientific Instruments·Shingi YamaguchiJunichiro Shiomi
Nov 13, 2017·ACS Biomaterials Science & Engineering·Harishankar Natesan, John C Bischof

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.