Thermal Destabilization of Collagen Matrix Hierarchical Structure by Freeze/Thaw

PloS One
Altug Ozcelikkale, Bumsoo Han

Abstract

This study aims to characterize and understand the effects of freezing on collagen structures and functionality. Specifically, thermodynamic destabilization of collagen at molecular- and fibril-levels by combination of low temperatures and freezing were experimentally characterized using modulated differential scanning calorimetry. In order to delineate the effects of sub-zero temperature and water-ice phase change, we hypothesized that the extent of destabilization can be determined based on post-thaw heat induced thermal denaturation of collagen. It is found that thermal denaturation temperature of collagen in hydrogel decreases by 1.4-1.6°C after freeze/thaw while no such decrease is observed in the case of molecular solution. The destabilization is predominantly due to ice formation. Exposure to low temperatures in the absence of ice has only minimal effect. Calorimetry measurements combined with morphological examination of collagen matrices by scanning electron microscopy suggest that freezing results in destabilization of collagen fibrils due to expansion of intrafibrillar space by ice formation. This fibril-level damage can be alleviated by use of cryoprotectant DMSO at concentrations as low as 0.5 M. A theoretical mode...Continue Reading

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Citations

May 2, 2019·Journal of Materials Science. Materials in Medicine·Kazim K MoncalIbrahim T Ozbolat
Oct 6, 2017·Journal of the Royal Society, Interface·Altug OzcelikkaleBumsoo Han
Nov 14, 2019·Veterinary Surgery : VS·Daniel J DuffyGeorge E Moore
Oct 27, 2017·Annals of the New York Academy of Sciences·Andrea H Lee, Dawn M Elliott
Jan 12, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ugo MasulloMarta Madaghiele
Mar 9, 2021·Tissue Engineering. Part C, Methods·M Gabriela EspinosaKyriacos A Athanasiou
May 2, 2021·Journal of the Mechanical Behavior of Biomedical Materials·Connor V CunnaneJohn J E Mulvihill
Jun 2, 2021·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Kameel ZunigaMarissa Nichole Rylander
Sep 14, 2021·ACS Biomaterials Science & Engineering·Hossein RavanbakhshYu Shrike Zhang

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Methods Mentioned

BETA
differential scanning calorimetry
differential scanning
scanning electron microscopy
biopsy
protein folding

Software Mentioned

ImageJ
MATLAB

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