PMID: 109478Mar 1, 1979

Thermal inactivation of a heat-resistant lipase produced by the psychotrophic bacterium Pseudomonas fluorescens

Journal of Dairy Science
R E AnderssonU Jonsson


Lipase from Pseudomonas fluorescens was studied for thermostability at temperatures ranging from 100 C to 160 C. The heat treatments were in two media, and heating times necessary to inactivate 90% of the enzyme at constant temperature were extremely long even at high temperatures, e.g. 3.6 min at 140 C in nutrient broth and 2.0 min at 170 C in skim milk. The increments of temperature to reduce these heating times 90% were 37.0 C in nutrient broth and 38.9 C in skim milk. The lipase was inactivated only partly after 20 h at 20 C in 8 M urea, 6 M guanidine hydrochloride, and 1.0% sodium dodecyl sulfate. Four percent 2-mercaptoethanol showed no effect.


Jun 1, 1975·Journal of Dairy Science·D M AdamsM L Speck
Apr 14, 1976·Biochimica Et Biophysica Acta·J Clarke, L M Shannon
Jan 1, 1971·Annual Review of Biochemistry·C Frieden
Jan 1, 1973·Archiv für Mikrobiologie·H ChanderB Ranganathan
Jan 11, 1967·Biochimica Et Biophysica Acta·I M KhanK M Shahani


Nov 11, 2011·Applied Biochemistry and Biotechnology·Abhishek Kumar Singh, Mausumi Mukhopadhyay
Oct 9, 2012·Applied and Environmental Microbiology·Myunghan SonJung Hoon Ahn
May 1, 1987·The Journal of Dairy Research·A R BuckyD S Robinson
Oct 1, 1985·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·F HaslbeckW Grosch
Aug 3, 2018·Journal of Zhejiang University. Science. B·Lei YuanGuo-Qing He

Related Concepts

Cell-Free System
Drug Stability
Protein Denaturation
Pseudomonas fluorescens

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