PMID: 43965Dec 11, 1979

Thermal perturbation differential spectra of ribonucleic acids. I. Hydration effects

Nucleic Acids Research
D FrechetP Remy

Abstract

A relatively important change in UV absorption is observed upon thermal perturbation of nucleotide solutions. Comparison of these thermal perturbation spectra of nucleic acid residues with solvent perturbation spectra of the same compounds suggests that this spectral change can most probably be attributed to temperature induced hydration change of the bases. This conclusion is confirmed by the results obtained from acid-base perturbation spectra of these nucleotides as well as thermal perturbation spectra of nucleotides containing modified bases. It is shown that this temperature dependent change in UV absorption is also present in dinucleoside monophosphates. In that case, this effect is superimposed upon the well known change in absorbance due to the unstacking of the bases during heating.

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R Cerf
Jan 1, 1972·Biopolymers·J T PowellW B Gratzer
Mar 14, 1972·Journal of Molecular Biology·M J Lowe, J A Schellman
Apr 1, 1966·Journal of Molecular Biology·S Z Hirschman, G FELSENFELD
Jan 1, 1971·Biopolymers·W C JohnsonJ C Girod
Sep 1, 1965·Journal of Molecular Biology·G FELSENFELD, S Z Hirschman
Jun 21, 1966·Biochemical and Biophysical Research Communications·S Hanlon
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Sep 1, 1964·Journal of Molecular Biology·H R MAHLERB D MEHROTRA

Related Concepts

Hydrogen-Ion Concentration
Oligoribonucleotides
Ribonucleotides
RNA
Spectrophotometry, Ultraviolet
Thermodynamics

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