Thermal proteome profiling for interrogating protein interactions.

Molecular Systems Biology
André MateusMikhail M Savitski

Abstract

Thermal proteome profiling (TPP) is based on the principle that, when subjected to heat, proteins denature and become insoluble. Proteins can change their thermal stability upon interactions with small molecules (such as drugs or metabolites), nucleic acids or other proteins, or upon post-translational modifications. TPP uses multiplexed quantitative mass spectrometry-based proteomics to monitor the melting profile of thousands of expressed proteins. Importantly, this approach can be performed in vitro, in situ, or in vivo. It has been successfully applied to identify targets and off-targets of drugs, or to study protein-metabolite and protein-protein interactions. Therefore, TPP provides a unique insight into protein state and interactions in their native context and at a proteome-wide level, allowing to study basic biological processes and their underlying mechanisms.

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Citations

Jun 4, 2020·Mass Spectrometry Reviews·Teck Yew LowMingliang Ye
Jul 28, 2020·Bioinformatics·Nils KurzawaMikhail M Savitski
Jul 4, 2020·Signal Transduction and Targeted Therapy·Zhe ZhangCanhua Huang
Jan 16, 2021·Journal of Zhejiang University. Science. B·Zhen Chen, Junjie Chen
Feb 13, 2021·Cell Chemical Biology·Louis P ConwayChristopher G Parker
Dec 11, 2020·Nature·André MateusMikhail M Savitski
Feb 17, 2021·Molecular Systems Biology·Joel SelkrigMikhail M Savitski
Feb 20, 2021·Journal of Proteome Research·Wout BittremieuxNuno Bandeira
Mar 5, 2021·Journal of Proteome Research·Neil A McCrackenAmber L Mosley
Mar 19, 2021·Molecular Systems Biology·Michael ZimmermannLisa Maier
Apr 10, 2021·Briefings in Bioinformatics·Yinyin WangJing Tang
May 28, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G Melissa KrishnathasIris Bischoff-Kont
May 18, 2021·Chembiochem : a European Journal of Chemical Biology·Kuan-Yi LuEmily R Derbyshire
Jun 19, 2021·Nature Methods·Clément M PotelMikhail M Savitski
Jun 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hong-Wei ZhangXin Luan
Jul 1, 2021·Communications Biology·Siqi FangOliver M Crook
Sep 4, 2021·Journal of Medicinal Chemistry·Ricardo A M SerafimMatthias Gehringer
Sep 22, 2021·Molecular Cell·Vidhi PareekStephen J Benkovic

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Methods Mentioned

BETA
thermal shift
gene
PISA
thermal

Software Mentioned

Proteome Discoverer
TPP
CETSA
TR
MaxQuant
Mascot

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