PMID: 7022139Jan 1, 1981Paper

Thermal resistance to photoreactivation of specific mutations potentiated in E. coli B/r ung by ultraviolet light

Molecular & General Genetics : MGG
D F Fix, R Bockrath

Abstract

Mutagenesis by ultraviolet light was studied in a strain of E. coli ung, which lacks uracil-DNA glycosylase activity. Mutation potentiated by UV in cells already induced by nalidixic acid treatment was still photoreversible suggesting that pyrimidine dimers act directly as premutational photoproducts. Secondly, irradiated cells were held in buffer at 48 degrees C for 0 to 135 min to allow for deamination of cytosines in pyrimidine dimers. The mutation frequencies for class 2 de novo suppressor mutation, for class 2 converted suppressor mutation and for backmutation were individually determined, before and after photoreactivation, as a function of this thermal treatment. Backmutation remained sensitive to photoreactivation throughout the treatment but de novo and converted suppressor mutations rapidly developed resistance to photoreactivation. This resistance was not seen in an ung+ control. A model is proposed to account for the selective resistance based on the hypothesis that class 2 de novo and converted suppressor mutations normally result from UV by GC to AT transitions at T = C dimers. The model described deamination of the cytosine residues in these dimers to become uracil residues. In consequence, monomerization by phot...Continue Reading

References

Apr 1, 1977·Photochemistry and Photobiology·R C Bockrath, J E Palmer
Nov 14, 1977·Molecular & General Genetics : MGG·R C Bockrath, J E Palmer
Mar 2, 1978·Nature·H R Warner, B K Duncan
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G VillaniM Radman
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·E M Witkin, I E Wermundsen
Dec 15, 1977·Journal of Molecular Biology·C Coulondre, J H Miller
Dec 1, 1976·Bacteriological Reviews·E M Witkin
May 1, 1966·Journal of Molecular Biology·R B Setlow, W L Carrier
Jan 1, 1973·Photochemistry and Photobiology·R M Tyrrell
Jul 1, 1973·Mutation Research·R Bockrath, M K Cheung
Nov 1, 1970·Mutation Research·M K Cheung, R C Bockrath
Nov 1, 1980·Mutation Research·R BockrathS Kristoff

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Citations

Sep 1, 1986·Molecular & General Genetics : MGG·R Bockrath, P Mosbaugh
May 1, 1993·Mutation Research·C W LawrenceB J Kilbey
Oct 10, 2006·Chemical Communications : Chem Comm·Kenzo FujimotoIsao Saito
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D Fix, R Bockrath
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·I TessmanM A Kennedy
Aug 25, 1993·Nucleic Acids Research·P E Gibbs, C W Lawrence
Nov 21, 2007·Mutation Research·Wai Bing Mak, Douglas Fix
Oct 30, 2014·Photochemistry and Photobiology·Douglas E Brash
Jun 3, 2000·Journal of Molecular Biology·J H YoonG P Pfeifer
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·Z LivnehT Elizur
Jul 16, 2010·Chembiochem : a European Journal of Chemical Biology·Kenzo FujimotoYoshinaga Yoshimura
Jun 1, 1992·Molecular & General Genetics : MGG·B A Bridges
May 1, 1997·Journal of Bacteriology·M J HorsfallC W Lawrence
Sep 1, 1994·Microbiological Reviews·C P Selby, A Sancar

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