Thermal stability of chicken brain α-spectrin repeat 17: a spectroscopic study.

Journal of Biomolecular NMR
Annette K BrennerArnt J Raae

Abstract

Spectrin is a rod-like multi-modular protein that is mainly composed of triple-helical repeats. These repeats show very similar 3D-structures but variable conformational and thermodynamical stabilities, which may be of great importance for the flexibility and dynamic behaviour of spectrin in the cell. For instance, repeat 17 (R17) of the chicken brain spectrin α-chain is four times less stable than neighbouring repeat 16 (R16) in terms of ∆G. The structure of spectrin repeats has mainly been investigated by X-ray crystallography, but the structures of a few repeats, e.g. R16, have also been determined by NMR spectroscopy. Here, we undertook a detailed characterization of the neighbouring R17 by NMR spectroscopy. We assigned most backbone resonances and observed NOE restraints, relaxation values and coupling constants that all indicated that the fold of R17 is highly similar to that of R16, in agreement with previous X-ray analysis of a tandem repeat of the two domains. However, (15)N heteronuclear NMR spectra measured at different temperatures revealed particular features of the R17 domain that might contribute to its lower stability. Conformational exchange appeared to alter the linker connecting R17 to R16 as well as the BC-l...Continue Reading

References

Oct 29, 1992·Nature·A MusacchioM Saraste
Nov 20, 1991·Journal of Molecular Biology·D S WishartF M Richards
Jan 1, 1989·Life Sciences·L W FungM E Johnson
Mar 3, 1986·FEBS Letters·L W FungM E Johnson
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·R I MacDonaldM Saraste
Jan 1, 1993·Annual Review of Cell Biology·V Bennett, D M Gilligan
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Sep 1, 1995·Journal of Biomolecular NMR·D S WishartB D Sykes
Apr 1, 1996·FEBS Letters·J PascualM Saraste
Mar 1, 1997·Nature Structural Biology·K Djinovic CarugoM Saraste
Oct 21, 1999·Journal of Magnetic Resonance·M SchubertH Oschkinat
Jan 3, 2001·Journal of Magnetic Resonance·M SchubertP Schmieder
Dec 13, 2001·Journal of Magnetic Resonance·M SchubertP Schmieder
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Yunjun Wang, Oleg Jardetzky
Apr 4, 2003·The Journal of Biological Chemistry·Sunghyouk ParkLeslie W-M Fung
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jul 10, 2004·Journal of Biomolecular NMR·Kasper KowalskiGrant W Booker
Nov 4, 2004·Journal of Molecular Biology·Hideki KusunokiAlfonso Mondragón
Jan 3, 2006·Biophysical Journal·Sarah BateyJane Clarke
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
Oct 1, 1999·Journal of Biomolecular NMR·T DiercksH Kessler
Mar 1, 1994·Journal of Biomolecular NMR·R WeisemannC Griesinger

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Citations

Dec 5, 2013·Science China. Life Sciences·Rui ZhangDongHai Li
Aug 14, 2015·Journal of Molecular Recognition : JMR·Line M MyklebustArnt J Raae
May 16, 2013·Biochimica Et Biophysica Acta·Beata MachnickaAleksander F Sikorski
Apr 28, 2020·Journal of the American Chemical Society·Or SzekelyLucio Frydman

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