Thermal stability of cytochrome c' from mesophilic Shewanella amazonensis

Bioscience, Biotechnology, and Biochemistry
Yuki KatoYoshihiro Sambongi

Abstract

Cytochrome c' (SACP) from mesophilic Shewanella amazonensis, growing optimally at 37 °C, was thermally more stable than cytochrome c' (AVCP) from mesophilic Allochromatium vinosum, growing optimally at 25 °C. In contrast, SACP was less stable than cytochrome c' (PHCP) from thermophilic Hydrogenophilus thermoluteolus, growing optimally at 52 °C. Although only 28% of the SACP amino acid sequence was identical to those of AVCP and PHCP, the latter two being 55% identical, the overall main chain structures of the three cytochromes c' were similar, and SACP exhibited thermal stability intermediate between those of AVCP and PHCP. For these three proteins, the higher the stability is, the lesser the number of Gly residues in the putative α-helical regions is. Cytochromes c' including the present three are suitable for examining the protein stabilization mechanisms, because they are structurally similar and available from environments with a wide range of temperatures.

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Jun 1, 1970·Applied Microbiology·H W Holm, J W Vennes
Sep 12, 1998·International Journal of Systematic Bacteriology·K VenkateswaranK H Nealson
Sep 8, 2009·Bioscience, Biotechnology, and Biochemistry·Masaru YamanakaYoshihiro Sambongi
May 13, 2010·Bioscience, Biotechnology, and Biochemistry·Satoshi TakenakaYoshihiro Sambongi
Aug 9, 2013·Bioscience, Biotechnology, and Biochemistry·Sotaro FujiiYoshihiro Sambongi
May 2, 2014·Nucleic Acids Research·Marco BiasiniTorsten Schwede
Sep 18, 2014·Bioscience, Biotechnology, and Biochemistry·Sotaro FujiiYoshihiro Sambongi

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Citations

Jan 18, 2017·Protein Science : a Publication of the Protein Society·Sotaro FujiiYoshihiro Sambongi
Nov 2, 2018·Bioscience, Biotechnology, and Biochemistry·Satoshi Wakai
Jan 5, 2017·Extremophiles : Life Under Extreme Conditions·Taka-Aki KuribayashiYoshihiro Sambongi
Jun 15, 2021·Bioscience, Biotechnology, and Biochemistry·Sotaro FujiiYoshihiro Sambongi

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Methods Mentioned

BETA
gel filtration
protein assay
column
electrophoresis
circular dichroism
column chromatography
X-ray

Software Mentioned

SWISS
MODEL
BLAST

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