PMID: 11606207Oct 19, 2001Paper

Thermal unfolding of monomeric and dimeric beta-lactoglobulins

European Journal of Biochemistry
D FessasFrancesco Bonomi

Abstract

The thermal stabilities of dimeric bovine beta-lactoglobulin and monomeric equine beta-lactoglobulin were investigated at neutral pH by means of differential scanning calorimetry, circular dichroism, tryptophan fluorescence, and by binding of an hydrophobic probe. Differential scanning calorimetry showed the presence of two structural domains with different thermal stabilities in both proteins. Thermodynamic analysis of the calorimetric signal revealed that the two domains unfold independently according to a mechanism where an equilibrium step is followed by an irreversible transition. The spectroscopic data supported this model and allowed recognition of the structural regions corresponding to the more thermally stable domain. The differences in thermal stability between the two proteins can be primarily ascribed to the properties of the less stable domain.

References

Sep 22, 1992·Biochemistry·Y V Griko, P L Privalov
Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·S W Englander, L Mayne
Jul 16, 1985·Biochemistry·S P ManlyJ M Sturtevant
Jan 1, 1974·CRC Critical Reviews in Biochemistry·E H Strickland
Oct 10, 1972·Biochemistry·K A Robillard, A Wishnia
Dec 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A ContiG Braunitzer
Sep 1, 1995·Trends in Biochemical Sciences·R A Sayle, E J Milner-White
Dec 15, 1994·European Journal of Biochemistry·S P Roefs, K G De Kruif
Jul 1, 1994·Biophysical Journal· Griko YuV, V P Kutyshenko
Apr 1, 1994·Journal of Protein Chemistry·S CairoliF Bonomi
Aug 1, 1994·The Journal of Dairy Research·F BonomiG Solaroli
Jun 1, 1997·Journal of Dairy Science·Q WangH E Swaisgood
Jun 1, 1997·International Journal of Biological Macromolecules·C GiancolaG Barone
Feb 19, 1998·FEBS Letters·M VerheulK G de Kruif
Dec 29, 1998·The Journal of Biological Chemistry·S Y WuL Sawyer
Mar 10, 1999·Biopolymers·M VerheulK G de Kruif

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Citations

May 27, 2011·Amino Acids·Ivano EberiniElisabetta Gianazza
Apr 22, 2011·Journal of Agricultural and Food Chemistry·Alberto BarbiroliStefania Iametti
Mar 25, 2011·Journal of Agricultural and Food Chemistry·Phoebe X Qi, Charles I Onwulata
Jun 4, 2008·The Journal of Physical Chemistry. B·Nicholas F FernandezLorenzo Brancaleon
Dec 8, 2006·The European Physical Journal. E, Soft Matter·E H C BromleyA M Donald
Nov 19, 2009·Biological Chemistry·Alberto BarbiroliMaria Caterina Vilardo
Feb 18, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Alice ChevalleyMichèle Salmain
Apr 16, 2014·Food Chemistry·Adrián A PerezLiliana G Santiago
Apr 30, 2003·Protein Science : a Publication of the Protein Society·Loredano PollegioniFrancesco Bonomi
Apr 13, 2004·Protein Science : a Publication of the Protein Society·Thomas CroguennecSaïd Bouhallab
Sep 29, 2011·Molecular Nutrition & Food Research·Mauro MarengoHanne Frokiaer
Feb 22, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Ewa PeckaKatarzyna Czyż
Mar 20, 2016·European Biophysics Journal : EBJ·Liliana LighezanAdrian Neagu
Apr 25, 2006·Biophysical Journal·Semen O YesylevskyyAlexander P Demchenko
Feb 24, 2006·Biophysical Chemistry·Xiaohua LiuErkang Wang
Aug 9, 2005·Biochimica Et Biophysica Acta·Andrea StirpeLuigi Sportelli
Jul 26, 2015·International Journal of Biological Macromolecules·Mazen Haj SleimanMaria Micha-Screttas
Nov 2, 2013·Food Chemistry·Osvaldo E SpontonLiliana G Santiago
Oct 25, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Iuliana AproduNicoleta Stănciuc
Dec 25, 2004·Biophysical Chemistry·Valeria Vetri, Valeria Militello
Oct 4, 2005·International Journal of Biological Macromolecules·Annemarie M M van TeeffelenHarmen H J de Jongh
Jun 14, 2006·Journal of Mass Spectrometry : JMS·Gaetano InvernizziRita Grandori
Jul 11, 2006·Biopolymers·Claude R Gicquaud, Benoit Heppell
Apr 9, 2005·The Journal of Biological Chemistry·Laura CaldinelliLoredano Pollegioni
Feb 11, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Francesco BonomiAnna Morleo
Sep 15, 2020·PloS One·Ícaro M L G BarretoAdriano H N Rangel
Jul 7, 2011·Biopolymers·Asghar Taheri-KafraniThomas Haertlé
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria-Beatrice ColtelliAndrea Lazzeri
Dec 11, 2007·Biomacromolecules·Li LiangMuriel Subirade
Dec 1, 2007·International Journal of Biological Macromolecules·Alessio CoiMaria Luisa Ganadu
Jun 10, 2004·Journal of Agricultural and Food Chemistry·Guoyan ZhangCharles C Hardin
Aug 21, 2007·Journal of Proteome Research·Zhijuan BaoHuimin Ma
Feb 16, 2007·The Journal of Physical Chemistry. B·Billie J HarveyLorenzo Brancaleon

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