PMID: 6990416Apr 1, 1980Paper

Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27

Proceedings of the National Academy of Sciences of the United States of America
I KumagaiT Oshima

Abstract

The contents of 2'-O-methylguanosine and 1-methyladenosine in unfractionated tRNA obtained from Thermus thermophilus HB27 were found to increase significantly when the bacterium was grown at a higher temperature (80 degrees C). S-Adenosyl-L-methionine-dependent tRNA (guanosine-2')-methyltransferase (EC 2.1.1.34) and tRNA (adenine-1)-methyltransferase (EC 2.1.1.36) were detected in a cell-free extract of the thermophile, and both of them were partially purified. tRNA (guanosine-2')-methyltransferase specifically catalyzed the methylation of the guanylate residue at position 19 from the 5' end of Escherichia coli tRNAMetf. The amounts of these methyltransferases in the cells and their thermal characteristics seemed to be independent of the growth temperature of the bacterial cells from which the enzymes were extracted. It was inferred that the temperature dependence of the methylation process in vivo is accounted for, not by temperature dependence of enzyme formation, but by that of the enzyme activity.

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Citations

Feb 9, 2012·The Journal of Experimental Medicine·Stefanie GehrigMark Helm
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Elizabeth PlesheRobert T Batey
Dec 15, 1982·European Journal of Biochemistry·I A MorozovT V Venkstern
May 18, 2016·Bioscience, Biotechnology, and Biochemistry·Yuuki MitsukawaJun Ogawa
Apr 5, 2017·Biomolecules·Christian LorenzMario Mörl
Sep 25, 1998·The Journal of Biological Chemistry·H HoriK Watanabe
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroyuki HoriKimitsuna Watanabe
Aug 9, 2011·Wiley Interdisciplinary Reviews. RNA·Yuri Motorin, Mark Helm
Sep 8, 2018·European Journal of Mass Spectrometry·Zachary J DevereauxM T Rodgers
Aug 15, 2002·The Journal of Biological Chemistry·Naoki ShigiKimitsuna Watanabe
Dec 1, 2005·The Journal of Biological Chemistry·Naoki ShigiKimitsuna Watanabe
Jan 8, 2005·The Journal of Biological Chemistry·Kazunori WatanabeHiroyuki Hori
Jul 18, 2014·Genomics & Informatics·Mayavan SubramanianDorairaj Sudarsanam
Aug 14, 2020·Genes·Ashley M EdwardsPatricia C Dos Santos
Sep 12, 2006·The Journal of Biological Chemistry·Kazunori WatanabeHiroyuki Hori
May 13, 2010·Biochemistry·Yuri Motorin, Mark Helm

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