Thermally induced osteocyte damage initiates a remodelling signaling cascade

PloS One
E B DolanLaoise M McNamara

Abstract

Thermal elevations experienced by bone during orthopaedic procedures, such as cutting and drilling, exothermal reactions from bone cement, and thermal therapies such as tumor ablation, can result in thermal damage leading to death of native bone cells (osteocytes, osteoblasts, osteoclasts and mesenchymal stem cells). Osteocytes are believed to be the orchestrators of bone remodeling, which recruit nearby osteoclast and osteoblasts to control resorption and bone growth in response to mechanical stimuli and physical damage. However, whether heat-induced osteocyte damage can directly elicit bone remodelling has yet to be determined. This study establishes the link between osteocyte thermal damage and the remodeling cascade. We show that osteocytes directly exposed to thermal elevations (47°C for 1 minute) become significantly apoptotic and alter the expression of osteogenic genes (Opg and Cox2). The Rankl/Opg ratio is consistently down-regulated, at days 1, 3 and 7 in MLO-Y4s heat-treated to 47°C for 1 minute. Additionally, the pro-osteoblastogenic signaling marker Cox2 is significantly up-regulated in heat-treated MLO-Y4s by day 7. Furthermore, secreted factors from heat-treated MLO-Y4s administered to MSCs using a novel co-cultu...Continue Reading

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Citations

Aug 4, 2015·Current Rheumatology Reports·Alesha B Castillo, Philipp Leucht
Jun 24, 2016·Journal of the Royal Society, Interface·Eimear B DolanLaoise M McNamara
Jan 8, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sean McCutcheonMaribel Vazquez
Sep 1, 2020·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Andrei Fernandes JoaquimK Daniel Riew
Apr 25, 2016·Hormone Molecular Biology and Clinical Investigation·Alfredo CapparielloNadia Rucci
Jun 10, 2020·International Journal of Molecular Sciences·Kosuke HamajimaTakahiro Ogawa
Dec 6, 2017·Journal of Dental Research·C-H ChenJ A Helms
Feb 26, 2021·Clinical Oral Implants Research·Raphael HeuzerothSebastian Kühl

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Methods Mentioned

BETA
flow cytometry
DNA assay

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