PMID: 15386965Sep 25, 2004Paper

Thermally-reversible gel for 3-D cell culture of chondrocytes

Journal of Materials Science. Materials in Medicine
M JasionowskiA Gutowska

Abstract

Regeneration of destroyed articular cartilage can be induced by transplantation of cartilage cells into a defect. The best results are obtained by the use of autologus cells. However, obtaining large amounts of autologus cartilage cells causes a problem of creating a large cartilage defect in a donor site. Techniques are currently being developed to harvest a small number of cells and propagate them in vitro. It is a challenging task, however, due to the fact that ordinarily, in a cell culture on flat surfaces, chondrocytes do not maintain their in vivo phenotype and irreversibly diminish or cease the synthesis of the phenotypic markers for articular chondrocytes. Therefore, the research is continuing to develop culture conditions for chondrocytes with the preserved phenotype. We have investigated the use of thermoreversible gelling polymer based on N-isopropylacrylamide for the in vitro cell culture of chondrocytes.

Citations

Jul 22, 2011·Journal of the Royal Society, Interface·James W LapworthStephen Rimmer
Oct 9, 2014·Biomacromolecules·Jian YangGuillermo A Ameer
Aug 12, 2008·Journal of Bioscience and Bioengineering·Keun Hong Park, Kun Na
May 31, 2016·Genes & Diseases·Viktor TollemarRussell R Reid
Aug 1, 2009·IEEE Transactions on Biomedical Circuits and Systems·J Flueckiger, K C Cheung

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