Thermally Stable Ionic Liquid-Based Microemulsions for High-Temperature Stabilization of Lysozyme at Nanointerfaces

Langmuir : the ACS Journal of Surfaces and Colloids
Manvir KaurTejwant Singh Kang

Abstract

The development of new strategies for thermal stability and storage of enzymes is very important, considering the nonretention of catalytic activity by enzymes under harsh conditions of temperature. Following this, herein, a new approach based on the interfacial adsorption of lysozyme (LYZ) at nanointerfaces of ionic liquid (IL)-based microemulsions, for enhanced thermal stability of LYZ, is reported. Microemulsions (MEs) composed of dialkyl imidazolium-based surface active ILs (SAILs) as surfactants, ILs as the nonpolar phase, and ethylene glycol (EG) as the polar phase, without any cosurfactants, have been prepared and characterized in detail. Various regions corresponding to polar-in-IL, bicontinuous, and IL-in-polar phases have been characterized using conductivity measurements. Dynamic light scattering (DLS) measurements have provided insights into the size distribution of microdroplets, whereas temperature-dependent DLS measurements established the thermal stability of the MEs. Nanointerfaces formed by SAILs with EG in thermally stable reverse MEs act as fluid scaffolds to adsorb and provide thermal stability, up to 120 °C, to LYZ. Thermally treated LYZ upon extraction into a buffer shows enzyme activity owing to negligib...Continue Reading

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Citations

Dec 22, 2020·Physical Chemistry Chemical Physics : PCCP·Manvir KaurTejwant Singh Kang
Jul 11, 2021·Advanced Science·Alexander M CurreriEden E L Tanner
Jul 9, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Xue WangSergiy Minko
Jan 4, 2022·Langmuir : the ACS Journal of Surfaces and Colloids·Sima LashkariMichael A Pope

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