Thermodynamic analysis of the heterodimerization of leucine zippers of Jun and Fos transcription factors

Biochemical and Biophysical Research Communications
Kenneth L SeldeenAmjad Farooq

Abstract

Jun and Fos are components of the AP1 family of transcription factors and bind to the promoters of a diverse multitude of genes involved in critical cellular responses such as cell growth and proliferation, cell cycle regulation, embryonic development and cancer. Here, using the powerful technique of isothermal titration calorimetry, we characterize the thermodynamics of heterodimerization of leucine zippers of Jun and Fos. Our data suggest that the heterodimerization of leucine zippers is driven by enthalpic forces with unfavorable entropy change at physiological temperatures. Furthermore, the basic regions appear to modulate the heterodimerization of leucine zippers and may undergo at least partial folding upon heterodimerization. Large negative heat capacity changes accompanying the heterodimerization of leucine zippers are consistent with the view that leucine zippers do not retain alpha-helical conformations in isolation and that the formation of the native coiled-coil alpha-helical dimer is attained through a coupled folding-dimerization mechanism.

References

Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R S SpolarM T Record
Nov 4, 1988·Cell·T Curran, B R Franza
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·L R PatelT K Kerppola
Mar 5, 2008·Archives of Biochemistry and Biophysics·Kenneth L SeldeenAmjad Farooq

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Citations

Jan 5, 2011·The FEBS Journal·Jonathan A R Worrall, Jody M Mason
Mar 10, 2010·Journal of Molecular Recognition : JMR·Robert J FalconerBrett M Collins
Sep 12, 2009·Biomacromolecules·Erinc Sahin, Kristi L Kiick
Aug 7, 2013·Biochemistry·Ariam I KidaneElisar Barbar

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