Thermodynamic and kinetic framework of selenocysteyl-tRNASec recognition by elongation factor SelB.

The Journal of Biological Chemistry
Alena PaleskavaMarina V Rodnina

Abstract

SelB is a specialized translation elongation factor that delivers selenocysteyl-tRNA(Sec) (Sec-tRNA(Sec)) to the ribosome. Here we show that Sec-tRNA(Sec) binds to SelB.GTP with an extraordinary high affinity (K(d) = 0.2 pm). The tight binding is driven enthalpically and involves the net formation of four ion pairs, three of which may involve the Sec residue. The dissociation of tRNA from the ternary complex SelB.GTP.Sec-tRNA(Sec) is very slow (0.3 h(-1)), and GTP hydrolysis accelerates the release of Sec-tRNA(Sec) by more than a million-fold (to 240 s(-1)). The affinities of Sec-tRNA(Sec) to SelB in the GDP or apoforms, or Ser-tRNA(Sec) and tRNA(Sec) to SelB in any form, are similar (K(d) = 0.5 microm). Thermodynamic coupling in binding of Sec-tRNA(Sec) and GTP to SelB ensures at the same time the specificity of Sec- versus Ser-tRNA(Sec) selection and rapid release of Sec-tRNA(Sec) from SelB after GTP cleavage on the ribosome. SelB provides an example for the evolution of a highly specialized protein-RNA complex toward recognition of unique set of identity elements. The mode of tRNA recognition by SelB is reminiscent of another specialized factor, eIF2, rather than of EF-Tu, the common delivery factor for all other aminoacyl-t...Continue Reading

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Citations

Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jared M SchraderOlke C Uhlenbeck
Jun 29, 2012·The Journal of Biological Chemistry·Alena PaleskavaMarina V Rodnina
Sep 21, 2012·The Journal of Biological Chemistry·Jonathan N Gonzalez-FloresPaul R Copeland
Feb 25, 2014·The Journal of Chemical Physics·Robert C HarrisMarcia O Fenley
Nov 12, 2013·Nucleic Acids Research·Frank PeskeWolfgang Wintermeyer
Jul 30, 2014·Nucleic Acids Research·Ken-ichi HarunaMarkus Englert
Aug 26, 2015·Nucleic Acids Research·Yuzuru ItohShigeyuki Yokoyama
Apr 4, 2016·Journal of Molecular Biology·Marina V Rodnina, Wolfgang Wintermeyer
Jun 5, 2015·Nucleic Acids Research·Suresh Babu KotiniMarina V Rodnina
Dec 19, 2014·Journal of the American Chemical Society·Ross ThyerAndrew D Ellington
Feb 1, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marina V RodninaHolger Stark
Sep 17, 2014·The EMBO Journal·Bernhard Kuhle, Ralf Ficner
Sep 13, 2019·Nucleic Acids Research·Marina V RodninaFrank Peske
Aug 1, 2013·Biomolecular Concepts·Jonathan N Gonzalez-FloresPaul R Copeland
May 21, 2015·The Journal of Biological Chemistry·Adrián García-MárquezNuria Sánchez-Puig
Dec 8, 2020·Biochemistry. Biokhimii︠a︡·A PaleskavaA L Konevega

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