Thermodynamic characterization of a triheme cytochrome family from Geobacter sulfurreducens reveals mechanistic and functional diversity.

Biophysical Journal
Leonor MorgadoCarlos A Salgueiro

Abstract

A family of five periplasmic triheme cytochromes (PpcA-E) was identified in Geobacter sulfurreducens, where they play a crucial role by driving electron transfer from the cytoplasm to the cell exterior and assisting the reduction of extracellular acceptors. The thermodynamic characterization of PpcA using NMR and visible spectroscopies was previously achieved under experimental conditions identical to those used for the triheme cytochrome c(7) from Desulfuromonas acetoxidans. Under such conditions, attempts to obtain NMR data were complicated by the relatively fast intermolecular electron exchange. This work reports the detailed thermodynamic characterization of PpcB, PpcD, and PpcE under optimal experimental conditions. The thermodynamic characterization of PpcA was redone under these new conditions to allow a proper comparison of the redox properties with those of other members of this family. The heme reduction potentials of the four proteins are negative, differ from each other, and cover different functional ranges. These reduction potentials are strongly modulated by heme-heme interactions and by interactions with protonated groups (the redox-Bohr effect) establishing different cooperative networks for each protein, which...Continue Reading

References

Dec 15, 1992·European Journal of Biochemistry·D L TurnerA V Xavier
Nov 1, 1996·European Journal of Biochemistry·D L TurnerA V Xavier
Dec 14, 1999·European Biophysics Journal : EBJ·G M Ullmann, E W Knapp
Apr 19, 2002·Nature·Susan E ChildersDerek R Lovley
Nov 9, 2002·European Journal of Biochemistry·Ilídio J CorreiaAntónio V Xavier
Sep 17, 2003·The Journal of Biological Chemistry·Philipp Voigt, Ernst-Walter Knapp
Mar 26, 2004·Nature Reviews. Microbiology·Derek R Lovley
Jun 24, 2005·Nature·Gemma RegueraDerek R Lovley
Sep 19, 2006·Nature Reviews. Microbiology·Karrie A WeberJohn D Coates
Mar 10, 2007·BMC Microbiology·Evgenya S ShelobolinaDerek R Lovley
Jun 23, 2007·Biochemical and Biophysical Research Communications·Leonor MorgadoCarlos A Salgueiro
Aug 19, 2007·Biochimica Et Biophysica Acta·Catarina M PaqueteTeresa Catarino
Jul 22, 2008·Biochimica Et Biophysica Acta·Yan-Huai R DingTünde Mester
Nov 13, 2008·Current Opinion in Biotechnology·Derek R Lovley
Nov 13, 2008·Proteins·Zhong Zheng, M R Gunner
Dec 3, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Bruno M FonsecaRicardo O Louro
Dec 17, 2008·Biochimica Et Biophysica Acta·Miguel PessanhaAntónio V Xavier

❮ Previous
Next ❯

Citations

Feb 5, 2013·Applied and Environmental Microbiology·Pravin Malla ShresthaDerek R Lovley
Aug 2, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Joana M DantasCarlos A Salgueiro
Nov 28, 2012·Biochemical Society Transactions·Pablo Sebastián BonanniJuan Pablo Busalmen
Sep 18, 2012·Biochimica Et Biophysica Acta·Fabian GreinInês A C Pereira
Feb 1, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Marisa R FerreiraCarlos A Salgueiro
Apr 24, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Telma C SantosCarlos A Salgueiro
Aug 31, 2016·Nature Reviews. Microbiology·Liang ShiJames K Fredrickson
Jun 23, 2018·FEMS Microbiology Ecology·Gemma Reguera
Jun 15, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ying LiuDaniel R Bond
Oct 21, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Joana M DantasRicardo O Louro
May 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Gemma Reguera
May 29, 2018·Microbial Biotechnology·Gemma Reguera
Dec 14, 2018·FEBS Open Bio·Marisa R FerreiraCarlos A Salgueiro
Sep 30, 2018·Applied and Environmental Microbiology·Bridget E ConleyJeffrey A Gralnick
May 20, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Joana M DantasCarlos A Salgueiro
Mar 21, 2021·Applied and Environmental Microbiology·Toshiyuki Ueki
Apr 30, 2021·The Journal of Biological Chemistry·Pilar C PortelaCarlos A Salgueiro
Jun 3, 2021·Antioxidants·Tomás M FernandesCarlos A Salgueiro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.