Thermodynamic characterization of an intermediate state of human growth hormone

Protein Science : a Publication of the Protein Society
I Gomez-OrellanaD R Paton

Abstract

The thermal denaturation of recombinant human growth hormone (rhGH) was studied by differential scanning calorimetry and circular dichroism spectroscopy (CD). The thermal unfolding is reversible only below pH 3.5, and under these conditions a single two-state transition was observed between 0 and 100 degrees C. The magnitudes of the deltaH and deltaCp of this transition indicate that it corresponds to a partial unfolding of rhGH. This is also supported by CD data, which show that significant secondary structure remains after the unfolding. Above pH 3.5 the thermal denaturation is irreversible due to the aggregation of rhGH upon unfolding. This aggregation is prevented in aqueous solutions of alcohols such as n-propanol, 2-propanol, or 1,2-propanediol (propylene glycol), which suggests that the self-association of rhGH is caused by hydrophobic interactions. In addition, it was found that the native state of rhGH is stable in relatively high concentrations of propylene glycol (up to 45% v/v at pH 7-8 or 30% at pH 3) and that under these conditions the thermal unfolding is cooperative and corresponds to a transition from the native state to a partially folded state, as observed at acidic pH in the absence of alcohols. In higher co...Continue Reading

References

Apr 3, 1979·Biochemistry·G Velicelebi, J M Sturtevant
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L Fu, E Freire
Sep 29, 1992·Biochemistry·S Bastiras, J C Wallace
Jan 1, 1992·Advances in Protein Chemistry·K P Murphy, E Freire
Oct 5, 1991·Journal of Molecular Biology·M F Jeng, S W Englander
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Aug 1, 1995·Proteins·J GómezE Freire
Oct 1, 1995·Nature Structural Biology·H Roder
Oct 1, 1995·Nature Structural Biology·J BalbachC M Dobson
Aug 25, 1995·The Journal of Biological Chemistry·K M YoungmanM R DeFelippis
Dec 1, 1994·Protein Science : a Publication of the Protein Society·D XieE Freire
Feb 22, 1995·Biochimica Et Biophysica Acta·M R DeFelippisH A Havel
Jan 1, 1993·Pharmaceutical Biotechnology·R Pearlman, T A Bewley
Feb 11, 1994·Journal of Molecular Biology·M H UltschA M de Vos
Feb 1, 1997·Protein Science : a Publication of the Protein Society·N HirotaY Goto

❮ Previous
Next ❯

Citations

Jan 15, 2005·Pharmaceutical Research·Sumitra TavornvipasKaneto Uekama
Jun 19, 2001·Biochimica Et Biophysica Acta·K ZhuP K Kinnunen
Jul 20, 2013·Analytical Chemistry·Caroline PritchardAlison E Ashcroft
Sep 26, 2002·FEBS Letters·Alexey A SchulgaMikhail P Kirpichnikov
Jan 21, 2011·Journal of Pharmaceutical Sciences·Filippo MulinacciTudor Arvinte
Sep 7, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Stefano SalmasoPaolo Caliceti
May 10, 2011·Biologicals : Journal of the International Association of Biological Standardization·Andrejus Cirkovas, Jolanta Sereikaite
Aug 28, 2010·Biologicals : Journal of the International Association of Biological Standardization·Mike Cauchy, Mary Alice Hefford
Mar 31, 2004·Diabetic Medicine : a Journal of the British Diabetic Association·M KidronH Bar-On
Apr 15, 2008·Proteins·Malene Ringkjøbing JensenJens J Led
Apr 3, 2015·Biotechnology Progress·Andrejus Cirkovas, Jolanta Sereikaite
Feb 17, 2006·Journal of Neurophysiology·Ghada S Mahmoud, Lawrence M Grover
Mar 17, 2018·Pharmaceutical Research·Vaishnavi Rohitkumar Shah, Pardeep K Gupta
Sep 10, 2002·Biologicals : Journal of the International Association of Biological Standardization·Mike CauchyMary Alice Hefford

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.