Thermodynamic characterization of the palm tree Roystonea regia peroxidase stability

Biochimie
Laura S ZamoranoV L Shnyrov

Abstract

The structural stability of a peroxidase, a dimeric protein from royal palm tree (Roystonea regia) leaves, has been characterized by high-sensitivity differential scanning calorimetry, circular dichroism, steady-state tryptophan fluorescence and analytical ultracentifugation under different solvent conditions. It is shown that the thermal and chemical (using guanidine hydrochloride (Gdn-HCl)) folding/unfolding of royal palm tree peroxidase (RPTP) at pH 7 is a reversible process involving a highly cooperative transition between the folded dimer and unfolded monomers, with a free stabilization energy of about 23 kcal per mol of monomer at 25 degrees C. The structural stability of RPTP is pH-dependent. At pH 3, where ion pairs have disappeared due to protonation, the thermally induced denaturation of RPTP is irreversible and strongly dependent upon the scan rate, suggesting that this process is under kinetic control. Moreover, thermally induced transitions at this pH value are dependent on the protein concentration, allowing it to be concluded that in solution RPTP behaves as dimer, which undergoes thermal denaturation coupled with dissociation. Analysis of the kinetic parameters of RPTP denaturation at pH 3 was accomplished on th...Continue Reading

References

Apr 15, 1987·Analytical Chemistry·R Q Thompson
Nov 1, 1987·Biopolymers·W J Becktel, J A Schellman
Jul 16, 1985·Biochemistry·S P ManlyJ M Sturtevant
Oct 1, 1995·Protein Science : a Publication of the Protein Society·J K MyersJ M Scholtz
May 25, 2002·European Journal of Biochemistry·Anabel RodríguezValery L Shnyrov
Sep 5, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Leandra WatanabeIgor Polikarpov

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Citations

Aug 7, 2013·International Journal of Biological Macromolecules·Irene Sánchez MoránEnrique Villar
Nov 10, 2011·Assay and Drug Development Technologies·Guillermo SenisterraMasoud Vedadi
Dec 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Larissa C TextorIgor Polikarpov
Apr 20, 2010·Archives of Biochemistry and Biophysics·Catherine MathéChristophe Dunand
Mar 5, 2010·Biophysical Chemistry·Jose M Sanchez-Ruiz
Sep 30, 2009·International Journal of Biological Macromolecules·Anna V KladovaSergey A Bursakov
May 12, 2009·International Journal of Biological Macromolecules·Laura S ZamoranoValery L Shnyrov
Feb 11, 2015·Biochimie·Amanda BernardesIgor Polikarpov
Sep 7, 2011·International Journal of Biological Macromolecules·Liliana M Moreno-VargasClaudia G Benítez-Cardoza
Sep 20, 2011·International Journal of Biological Macromolecules·Nazaret Hidalgo CuadradoValery L Shnyrov
Sep 24, 2014·International Journal of Biological Macromolecules·Patricia Perez GalendeValery L Shnyrov
May 20, 2017·Journal of Agricultural and Food Chemistry·Boting WenQing X Li
Jun 10, 2017·The Journal of Chemical Physics·Ying GuoElsa C Y Yan

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