Thermodynamic properties of triheme cytochrome PpcF from Geobacter metallireducens reveal unprecedented functional mechanism

Biochimica Et Biophysica Acta. Bioenergetics
Marisa R FerreiraCarlos A Salgueiro

Abstract

The bacterium Geobacter metallireducens is highly efficient in long-range extracellular electron transfer, a process that relies on an efficient bridging between the cytoplasmic electron donors and the extracellular acceptors. The periplasmic triheme cytochromes are crucial players in these processes and thus the understanding of their functional mechanism is crucial to elucidate the extracellular electron transfer processes in this microorganism. The triheme cytochrome PpcF from G. metallireducens has the lowest amino acid sequence identity with the remaining cytochromes from the PpcA-family of G. sulfurreducens and G. metallireducens, making it an interesting target for structural and functional studies. In this work, we performed a detailed functional and thermodynamic characterization of cytochrome PpcF by the complementary usage of NMR and visible spectroscopic techniques. The results obtained show that the heme reduction potentials are negative, different from each other and are also modulated by the redox and redox-Bohr interactions that assure unprecedented mechanistic features to the protein. The results showed that the order of oxidation of the hemes in cytochrome PpcF is maintained in the entire physiological pH rang...Continue Reading

References

Nov 1, 1996·European Journal of Biochemistry·D L TurnerA V Xavier
Jun 27, 2001·Applied and Environmental Microbiology·M V CoppiD R Lovley
Mar 26, 2004·Nature Reviews. Microbiology·Derek R Lovley
Jul 30, 2004·Biochimica Et Biophysica Acta·António V Xavier
May 9, 2006·Current Opinion in Biotechnology·Derek R Lovley
Mar 10, 2007·BMC Microbiology·Evgenya S ShelobolinaDerek R Lovley
Jul 22, 2008·Biochimica Et Biophysica Acta·Yan-Huai R DingTünde Mester
Nov 26, 2011·Advances in Microbial Physiology·Derek R LovleyKelly P Nevin
Feb 1, 2012·Environmental Microbiology Reports·Pier-Luc TremblayDerek Lovley
Apr 24, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Telma C SantosCarlos A Salgueiro
Jan 23, 2018·Acta Biomaterialia·Rhiannon C G CreaseyBronwyn Laycock
Feb 28, 2018·Archives of Biochemistry and Biophysics·Pilar C PortelaCarlos A Salgueiro
Sep 22, 2018·The Journal of Physical Chemistry. B·Nicole L IngAllon I Hochbaum
Dec 14, 2018·FEBS Open Bio·Marisa R FerreiraCarlos A Salgueiro
Jan 11, 2019·Bioelectronics in Medicine·Tom Guterman, Ehud Gazit
Oct 15, 2019·Frontiers in Microbiology·Derek R Lovley, David J F Walker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.