Thermodynamic signature of DNA damage: characterization of DNA with a 5-hydroxy-2'-deoxycytidine·2'-deoxyguanosine base pair.

Biochemistry
Manjori GangulyBarry Gold

Abstract

Oxidation of DNA due to exposure to reactive oxygen species is a major source of DNA damage. One of the oxidation lesions formed, 5-hydroxy-2'-deoxycytidine, has been shown to miscode by some replicative DNA polymerases but not by error prone polymerases capable of translesion synthesis. The 5-hydroxy-2'-deoxycytidine lesion is repaired by DNA glycosylases that require the 5-hydroxycytidine base to be extrahelical so it can enter into the enzyme's active site where it is excised off the DNA backbone to afford an abasic site. The thermodynamic and nuclear magnetic resonance results presented here describe the effect of a 5-hydroxy-2'-deoxycytidine·2'-deoxyguanosine base pair on the stability of two different DNA duplexes. The results demonstrate that the lesion is highly destabilizing and that the energy barrier for the unstacking of 5-hydroxy-2'-deoxycytidine from the DNA duplex may be low. This could provide a thermodynamic mode of adduct identification by DNA glycosylases that requires the lesion to be extrahelical.

References

Jul 27, 1992·FEBS Letters·B Halliwell, J M Gutteridge
Sep 1, 1992·Mutation Research·M Dizdaroglu
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R WagnerB N Ames
Jan 1, 1991·Free Radical Biology & Medicine·M Dizdaroglu
Apr 11, 1991·Nucleic Acids Research·Y OdaE Ohtsuka
Sep 1, 1990·Carcinogenesis·R A Floyd
Jun 3, 1988·Science·J A Imlay, S Linn
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C H LeeT Kakefuda
Oct 23, 1980·Nature·R WingR E Dickerson
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·L A LipscombL D Williams
Jul 29, 1994·Annals of the New York Academy of Sciences·G E Plum, K J Breslauer
Aug 30, 1996·The Journal of Biological Chemistry·E S HenleS Linn
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·D A Kreutzer, J M Essigmann
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·C D MolJ A Tainer
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·A KlunglandD E Barnes
Jun 20, 2000·Chemical Research in Toxicology·C J La FrancoisL C Sowers
Jun 28, 2002·Free Radical Biology & Medicine·Susan S Wallace
Jul 4, 2003·The EMBO Journal·J Christopher Fromme, Gregory L Verdine
Nov 26, 2003·Mutation Research·Jean CadetJean-Luc Ravanat
Dec 23, 2003·The Journal of Biological Chemistry·Patrick J O'Brien, Tom Ellenberger
Jun 11, 2004·Biophysical Journal·M BastosJ Teixeira
Sep 15, 2007·Nucleic Acids Research·Manjori GangulyBarry Gold
Nov 22, 2007·Nucleic Acids Symposium Series·Kazuo NegishiDavid Loakes
Nov 23, 2007·Nucleic Acids Research·Sébastien Tremblay, J Richard Wagner
Feb 6, 2008·Nucleic Acids Research·Sudarshana BasuSamit Adhya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.