Thermodynamics and kinetics of heat inactivation of a novel keratinase from Chryseobacterium sp. strain kr6

Applied Biochemistry and Biotechnology
Silvana Terra SilveiraA Brandelli

Abstract

A novel keratinase from Chryseobacterium sp. strain kr6 was purified to homogeneity by (NH(4))(2)SO(4) precipitation, gel permeation on Sephadex G-100, and Q-Sepharose Fast Flow anion-exchange chromatography. The molecular weight of the purified enzyme was around 20 kDa. Kinetic and thermodynamic parameters for thermal inactivation were determined. The influence of Ca(2+) and Mg(2+) ions and purification degree on the enzyme stability was evaluated in the range of 50 to 60 degrees C. The results showed that first-order kinetics explained well the thermal denaturation of the keratinase in this temperature interval. The presence of Ca(2+) increases significantly the enzyme stability. Compared with the controls, the half-life of the purified enzyme after two purification steps in the presence of Ca(2+) increased 7.3, 20.2, and 9.8 fold at 50, 55, and 60 degrees C, respectively. Thermodynamics parameters for thermal inactivation were also determined.

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Citations

Oct 19, 2012·Bioprocess and Biosystems Engineering·Voltaire Sant'AnnaAdriano Brandelli
May 31, 2012·Critical Reviews in Biotechnology·Rani GuptaQasim K Beg
Jun 7, 2020·Applied Biochemistry and Biotechnology·Andréia Monique LermenDaniel Joner Daroit
Oct 1, 2011·Applied Biochemistry and Biotechnology·Daniel Joner DaroitAdriano Brandelli
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Imania GhaffarJaved Iqbal Qazi

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