Thermodynamics of bovine spleen galectin-1 binding to disaccharides: correlation with structure and its effect on oligomerization at the denaturation temperature

Biochemistry
F P SchwarzGerardo R Vasta

Abstract

Isothermal titration calorimetry (ITC) measurements of the binding 1-beta carbohydrate-substituted galactopyranoside derivatives to galectin-1 from bovine spleen, a dimer with one binding site per subunit, were performed at 283-285 and 298 K. The disaccharides were lactose, methyl beta-lactoside, lactulose, 4-O-beta-D-galactopyranosyl-D-mannopyranoside, 3-O-beta-D-galactopyranosyl-D-arabinose, 2'-O-methyllactose, lacto-N-biose, N-acetyllactosamine, and thiodigalactopyranoside. The site binding enthalpies, DeltaHb, are the same at both temperatures and range from -42.2 +/- 3.3 kJ mol-1 for thiodigalactopyranoside to -24.5 +/- 0.5 kJ mol-1 for lacto-N-biose, and the site binding constants range from 4.86 +/- 0.78 x 10(3) M-1 for methyl beta-lactoside at 297.8 K to 6.54 +/- 0.97 x 10(4) M-1 for N-acetyllactosamine at 281.3 K. The binding reactions are enthalpically driven, exhibit enthalpy-entropy compensation, and, with the exception of N-acetyllactosamine, follow a van't Hoff dependence of the binding constant on temperature. The number of contacts at distances <4.0 A between the disaccharide and galectin was determined from the energy-minimized conformation of the complex derived from the X-ray crystallographic structure of the...Continue Reading

References

Jul 26, 1996·The Journal of Biological Chemistry·A SuroliaF P Schwarz

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Citations

May 22, 2003·Biochimica Et Biophysica Acta·María Mercedes IglesiasCarlota Wolfenstein-Todel
Aug 19, 2011·Biochemistry·Santiago Di LellaGabriel A Rabinovich
Jun 28, 2011·Journal of Chemical Information and Modeling·Carlos M A GuardiaDarío A Estrin
Jun 15, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kohei ShibaYoshiki Katayama
Apr 29, 2005·Organic & Biomolecular Chemistry·Moira AmbrosiBenjamin G Davis
Jun 11, 2011·Organic & Biomolecular Chemistry·Sonsoles Martín-SantamaríaHans-Joachim Gabius
Apr 3, 2010·Carbohydrate Research·Stefanie MeschBeat Ernst
Sep 15, 2012·Annals of the New York Academy of Sciences·Gerardo R VastaL Mario Amzel
Oct 7, 2004·Analytical Biochemistry·Pernilla SörmeHakon Leffler
Sep 3, 2004·Biochimica Et Biophysica Acta·Shyamasri Biswas, Arvind M Kayastha
Apr 26, 2011·Biochimica Et Biophysica Acta·Jun IwakiJun Hirabayashi
May 5, 2011·Biophysical Journal·Ignacia Echeverria, L Mario Amzel
Jun 10, 2014·The Journal of Physical Chemistry. B·Angelos ThanassoulasGilles Sigaud
Apr 28, 2017·Pathogens and Disease·Gerardo R VastaCheyenne Palm
Mar 24, 2005·British Journal of Cancer·G A Rabinovich
Oct 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura Poisa-BeiroBeatriz Novoa
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Jun 25, 2019·Frontiers in Immunology·Sandra M BloisGabriela Barrientos
Jul 15, 2006·Glycobiology·Isabelle CambyRobert Kiss
May 1, 2020·Developmental and Comparative Immunology·Gerardo R Vasta, Jin-Xing Wang

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