PMID: 7326May 1, 1976

Thermodynamics of hexokinase catalyzed reactions. II. Measurement and calculation of enthalpies of reaction as a function of magnesium ion concentration

Biophysical Chemistry
R N Goldberg


Enthalpies of phosphorylation of glucose by adenosine 5'-triphosphate have been measured as a function of concentrations of magnesium chloride in TRIS/TRIS-HCl buffer in the pH range 8.64 to 8.98. These measurements are compared with the results of calculations of these enthalpies that use a coupled equilibrium formalism with equilibrium data and enthalpy values selected from the literature. The experimental results span the range of magnesium ion concentrations 1 X 10(-6) to 0.3 mol alpha-1 and show a total variation in the enthalpy of reaction of almost 10 kJ mol-1, with the most exothermic reaction occurring at a magnesium ion concentration of 6.0 X 10(-4) mol alpha-1. The calculated enthalpies of reaction, except for the magnesium ion concentration range 4 X 10(-6) to 5 X 10(-4) mol alpha-1, are, within estimated uncertainty intervals (0.8 to 10.2 kJ mol-1), in agreement with the measured values.


Jan 1, 1977·Analytica Chimica Acta·C D McGlothlin, W C Purdy
May 1, 1974·Archives of Biochemistry and Biophysics·M S Mohan, G A Rechnitz


Jun 1, 1982·Journal of Bioenergetics and Biomembranes·J C SariA Crevat
Sep 23, 2016·Biochemistry·Florian MeurerChristoph Held

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Glucose, (beta-D)-Isomer
Hexokinase 2
Hydrogen-Ion Concentration

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