Thermodynamics of polyethylenimine-DNA binding and DNA condensation.

Biophysical Journal
Kuniharu Utsuno, Hasan Uludağ

Abstract

In this study, polyethylenimine (PEI) binding to DNA was examined by isothermal titration calorimetry. Two types of binding modes were found to describe the interactions between these polyelectrolytes in buffers and in water. One type of binding involves PEI binding to the DNA groove because the enthalpy change of this binding mode is positive, and PEI is deprotonated to bind to DNA. Another likely binding mode involves external binding of PEI to the DNA phosphate backbone, accompanied with DNA condensation. The enthalpy change is negative and PEI is protonated when it binds to DNA in this mode. The intrinsic enthalpy change of first binding mode is 1.1 kJ/mol and -0.88 kJ/mol for the second binding mode. This result implies that the PEI is rearranged from the groove to the phosphate backbone of DNA when DNA is condensed. The mechanism of DNA condensation caused by PEI is discussed in this study.

References

May 29, 1979·Biochemistry·R W Wilson, V A Bloomfield
Jan 1, 1995·Annual Review of Physical Chemistry·C F Anderson, M T Record
Jun 1, 1995·Bioorganic & Medicinal Chemistry·D RentzeperisL A Marky
Aug 1, 1997·Nucleic Acids Research·D D DunlapL Monaco
Jul 30, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·W T GodbeyA G Mikos
Apr 12, 2002·Journal of Pharmaceutical Sciences·Aravind Asokan, Moo J Cho
May 23, 2003·Journal of Pharmaceutical Sciences·Christopher M WiethoffC Russell Middaugh
Jul 29, 2003·Journal of Pharmaceutical Sciences·Sirirat ChoosakoonkriangC Russell Middaugh
Dec 13, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ying-lin Zhou, Yuan-zong Li
Nov 16, 2004·The Journal of Gene Medicine·Akin AkincRobert Langer
Jan 18, 2005·Biophysical Journal·Mayank M Patel, Thomas J Anchordoquy
Apr 9, 2005·Biotechnology and Bioengineering·Vikas K SharmaAlexander M Klibanov
Jun 15, 2006·The Journal of Physical Chemistry. B·Wankee KimKazunori Kataoka
Aug 21, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Lisa E PrevetteMatthew L Lynch
Mar 4, 2008·Chemical & Pharmaceutical Bulletin·Kuniharu Utsuno
Jul 9, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Lisa E PrevetteTheresa M Reineke
Apr 16, 2009·The Journal of Physical Chemistry. B·Hiroyuki TanakaTomoji Kawai
Jan 12, 2010·Biomacromolecules·Lisa E PrevetteTheresa M Reineke

❮ Previous
Next ❯

Citations

Aug 15, 2013·The Journal of Physical Chemistry. B·Tiia-Maaria KetolaElina Vuorimaa-Laukkanen
Jul 2, 2011·Nanomedicine·Feiran HuangJunghae Suh
Mar 25, 2011·Journal of Pharmacy & Bioallied Sciences·Sarantis Giatrellis, George Nounesis
Dec 24, 2013·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Mahdi KarimiHossein Naderi-Manesh
Dec 19, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Lindomar J C AlbuquerqueFernando Carlos Giacomelli
Jan 3, 2012·Biomaterials·Hamidreza M AliabadiHasan Uludağ
Jan 4, 2012·Journal of Molecular Recognition : JMR·Rajesh GhaiBrett M Collins
Jul 6, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kay MarquardtFrank Runkel
Aug 16, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Abhijeet P Pandey, Krutika K Sawant
Jan 22, 2015·Physical Chemistry Chemical Physics : PCCP·Hanne S AntilaMaria Sammalkorpi
Jul 20, 2017·Journal of Biomaterials Science. Polymer Edition·Deniz Sezlev BilecenVasif Hasirci
Nov 11, 2018·Journal of Computational Chemistry·Subhamoy Mahajan, Tian Tang
Oct 12, 2016·Chemical & Pharmaceutical Bulletin·Kuniharu UtsunoHasan Uludağ
Jun 5, 2020·Biomacromolecules·Chanel C LaJayachandran N Kizhakkedathu
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Hashanthi K Abeyratne-PereraPreethi L Chandran
Apr 7, 2015·The Journal of Physical Chemistry. B·Deniz Meneksedag-ErolHasan Uludağ
Sep 17, 2011·Biomacromolecules·Chongbo SunHasan Uludağ
Jun 12, 2014·The Journal of Physical Chemistry. B·Sampada BagaiTian Tang
Feb 14, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Bruno Dos Santos de MacedoLetícia Vitorazi
Sep 4, 2020·The Journal of Physical Chemistry. B·M Arif KhanBarbara L Knutson
Oct 9, 2021·Signal Transduction and Targeted Therapy·Wenjuan MaYunfeng Lin
Apr 11, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kei X TanMichael K Danquah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.