Thermodynamics of the interaction of human immunoglobulin E with its high-affinity receptor Fc epsilon RI

Biochemistry
M B KeownH J Gould

Abstract

We have employed isothermal titration calorimetry (ITC) and circular dichroism (CD) spectroscopy to characterize the binding of soluble fragments of IgE (IgE-Fc and Fc epsilon 3-4) to a soluble fragment of the high-affinity receptor Fc epsilon RI alpha-chain (sFc epsilon RI alpha). The thermodynamic parameters for the interaction of IgE-Fc and Fc epsilon 3-4 with sFc epsilon RI alpha, determined using ITC, confirm the earlier conclusion that the C epsilon 2 domain is not involved in the interaction and that the stoichiometry of both complexes is 1:1. For both IgE-Fc and Fc epsilon 3-4, the value of Delta H degrees is -36.9 +/- 4.6 kcal mol-1 at 37.3 degreesC and Delta Cp degrees is -820 +/- 120 cal mol-1 K-1. The temperature at which DeltaS degrees is zero is 284 +/- 1 K, indicating that the entropy contribution to the thermodynamics of association is unfavorable at physiological temperature. Of particular interest is the large value of Delta Cp degrees. The large surface area of IgE and Fc epsilon RI alpha that is implicated in complex formation from previous mutagenesis studies on the two proteins may account in part for the magnitude of Delta Cp degrees. Additional contributions may arise from hydration within the binding si...Continue Reading

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Citations

Feb 9, 2011·Current Allergy and Asthma Reports·Lanny J Rosenwasser
Mar 9, 2005·The Journal of Allergy and Clinical Immunology·Jill A PooleLanny J Rosenwasser
Apr 16, 2002·Chemosphere·Song-Sheng QuWen-Ying Gao
Apr 17, 2002·Molecular Immunology·Beth A Wurzburg, Theodore S Jardetzky
Jun 8, 1999·Annual Review of Immunology·J P Kinet
Jul 22, 2011·Journal of Signal Transduction·Rodrigo Orlandini de Castro
Nov 1, 2011·Journal of Molecular Recognition : JMR·Line Naomi LundPhaedria Marie St Hilaire
Jun 22, 2011·MAbs·Jantine E Bakema, Marjolein van Egmond
Feb 5, 2000·Nature Biotechnology·T W Chang
Mar 4, 2005·The Journal of Biological Chemistry·James HuntAndrew J Beavil
Jan 16, 2021·ImmunoTargets and Therapy·Melissa Maria Johanna van Gool, Marjolein van Egmond

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