Abstract
beta3-Adrenergic-stimulated thermogenesis in brown adipose tissue (BAT) is diminished with age. beta3-Adrenergic receptors are positively coupled to adenylyl cyclase in BAT. To determine whether thermo- genesis, in response to direct activation of adenylyl cyclase, is also impaired with age, we examined whole body oxygen consumption, mitochondrial guanosine diphosphate (GDP) binding and BAT mitochondrial uncoupling protein (UPC) mRNA levels in 4- and 24-month-old F-344 rats following forskolin administration. We also examined the forskolin-induced change in body temperature in 4-month-old rats. In some instances, the results were compared with administration of the specific beta3-adrenergic agonist, CGP-12177. Forskolin (3.5 mg/kg) increased oxygen consumption but decreased body temperature. In subsequent experiments the BAT was unilaterally denervated. In these rats, the forskolin-(1.8 mg/kg) stimulated increase in oxygen consumption was similar in young and old rats. Forskolin increased GDP binding and UCP mRNA levels in both the denervated and innervated BAT pads. The increases were equal or greater in the BAT from senescent rats. These findings, coupled with our previous report of an impaired CGP-12177-stimulated increase i...Continue Reading
References
Jul 1, 1992·Proceedings of the Society for Experimental Biology and Medicine·P J ScarpaceS E Borst
Jun 1, 1991·Canadian Journal of Physiology and Pharmacology·P J ScarpaceS E Borst
Oct 1, 1991·Pharmacology, Biochemistry, and Behavior·H J Carlisle, M J Stock
Feb 1, 1991·The American Journal of Physiology·N FarmanJ M Gasc
Sep 15, 1987·Biochemical and Biophysical Research Communications·M S KatzS R Boland
May 1, 1988·Journal of Gerontology·P J ScarpaceJ E Morley
Mar 1, 1989·Journal of Gerontology·R B McDonaldB A Horwitz
Mar 1, 1994·Journal of Gerontology·M P Sanghani, P J Scarpace
Feb 1, 1994·Proceedings of the Society for Experimental Biology and Medicine·P J ScarpaceN Tümer
Citations
Apr 11, 1997·European Journal of Pharmacology·N TümerP J Scarpace
Nov 19, 2002·Neuroscience·G LiP J Scarpace
Dec 29, 2000·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·P J ScarpaceN Tümer
Mar 15, 1997·Annals of the New York Academy of Sciences·P J Scarpace
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kendall M CarlinTracy L Bale
Mar 25, 2011·Archives of Physiology and Biochemistry·Rocio Vila-Bedmar, Sonia Fernández-Veledo
Oct 5, 2016·Best Practice & Research. Clinical Endocrinology & Metabolism·Denis P Blondin, André C Carpentier
Oct 30, 2016·Experimental Gerontology·I RostásM Balaskó
Aug 8, 1998·American Journal of Physical Anthropology·V BelkinE Kobyliansky
Oct 21, 2017·Nature Reviews. Endocrinology·Matthias J Betz, Sven Enerbäck
Jan 27, 2019·Scientific Reports·Haaglim ChoDarren R Williams
Jul 1, 1997·The American Journal of Physiology·P J ScarpaceN Tümer
Jun 1, 1997·The American Journal of Physiology·Y KenanM Naim
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Aug 4, 1998·The American Journal of Physiology·P J Scarpace, M Matheny
Apr 30, 1998·Physiological Reviews·M Florez-Duquet, R B McDonald