Thermogenetic neurostimulation with single-cell resolution

Nature Communications
Yulia G ErmakovaV V Belousov

Abstract

Thermogenetics is a promising innovative neurostimulation technique, which enables robust activation of neurons using thermosensitive transient receptor potential (TRP) cation channels. Broader application of this approach in neuroscience is, however, hindered by a limited variety of suitable ion channels, and by low spatial and temporal resolution of neuronal activation when TRP channels are activated by ambient temperature variations or chemical agonists. Here, we demonstrate rapid, robust and reproducible repeated activation of snake TRPA1 channels heterologously expressed in non-neuronal cells, mouse neurons and zebrafish neurons in vivo by infrared (IR) laser radiation. A fibre-optic probe that integrates a nitrogen-vacancy (NV) diamond quantum sensor with optical and microwave waveguide delivery enables thermometry with single-cell resolution, allowing neurons to be activated by exceptionally mild heating, thus preventing the damaging effects of excessive heat. The neuronal responses to the activation by IR laser radiation are fully characterized using Ca2+ imaging and electrophysiology, providing, for the first time, a complete framework for a thermogenetic manipulation of individual neurons using IR light.

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Citations

Oct 19, 2017·Journal of Biophotonics·M S PochechuevA M Zheltikov
Jul 12, 2018·Small·Hadrien M L RobertGuillaume Baffou
Feb 13, 2020·Nanoscale·Juyoung KwonHeon-Jin Choi
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Aug 11, 2021·Brain Stimulation·David Dominguez-ParedesKristen L Kozielski
Aug 21, 2021·Nanotechnology·Masazumi Fujiwara, Yutaka Shikano

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Datasets Mentioned

BETA
GU562967
GU562966

Methods Mentioned

BETA
transfection
fluorescence imaging
Fluorescence
biosensors
PCR

Software Mentioned

SPSS
OriginPro
Thermogenetics

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