Thermogladius calderae gen. nov., sp. nov., an anaerobic, hyperthermophilic crenarchaeote from a Kamchatka hot spring

International Journal of Systematic and Evolutionary Microbiology
Tatiana V KochetkovaAnna A Perevalova

Abstract

An obligately anaerobic, hyperthermophilic, organoheterotrophic archaeon, strain 1633T, was isolated from a terrestrial hot spring of the Uzon Caldera (Kamchatka Peninsula, Russia). Cells were regular cocci, 0.5-0.9 μm in diameter, with one flagellum. The temperature range for growth was 80-95°C, with an optimum at 84°C. Strain 1633T grew on yeast extract, beef extract, peptone, cellulose and cellobiose. No growth was detected on other sugars or carbohydrates, organic acids, or under autotrophic conditions. The only detected growth products were CO2, acetate, and H2. Growth rate was stimulated by elemental sulfur, which was reduced to hydrogen sulfide. In silico calculated G+C content of strain 1633T genomic DNA was 55.64 mol%. 16S rRNA gene sequence analysis placed the strain 1633T together with the non-validly published "Thermogladius shockii" strain WB1 in a separate genus-level cluster within the Desulfurococcaceae family. ANI results revealed 75.72% identity between 1633T and WB1. Based on these results we propose a novel genus and species, for which the name Thermogladius calderae gen. nov., sp. nov. (type strain 1633T=DSM 22663T=VKM B-2946T) is proposed.

References

Dec 1, 1992·Journal of Bioenergetics and Biomembranes·G D Sprott
Jan 13, 2001·International Journal of Systematic and Evolutionary Microbiology·M I ProkofevaE A Bonch-Osmolovskaya
Jan 2, 2003·International Journal of Systematic and Evolutionary Microbiology·T G SokolovaE A Bonch-Osmolovskaya
Apr 2, 1963·Biochimica Et Biophysica Acta·J B ADAMS
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
May 10, 2005·International Journal of Systematic and Evolutionary Microbiology·A A PerevalovaE A Bonch-Osmolovskaya
Jun 23, 2005·Extremophiles : Life Under Extreme Conditions·Maria I ProkofevaChristian Jeanthon
Jan 16, 2007·International Journal of Systematic and Evolutionary Microbiology·Johan GorisJames M Tiedje
Oct 14, 2008·Applied and Environmental Microbiology·Anna A PerevalovaAlexander V Lebedinsky
Jun 23, 2009·International Journal of Systematic and Evolutionary Microbiology·I V KublanovE A Bonch-Osmolovskaya
Oct 20, 2009·International Journal of Systematic and Evolutionary Microbiology·Anna A PerevalovaElizaveta A Bonch-Osmolovskaya
Dec 6, 2011·International Journal of Systematic and Evolutionary Microbiology·Ok-Sun KimJongsik Chun
Jul 31, 2012·Journal of Bacteriology·Andrey V MardanovKonstantin G Skryabin
Mar 28, 2013·Archaea : an International Microbiological Journal·Bernd WemheuerRolf Daniel
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Dec 17, 2014·International Journal of Systematic and Evolutionary Microbiology·G B SlobodkinaA I Slobodkin
Sep 10, 2015·Extremophiles : Life Under Extreme Conditions·Nikolay A ChernyhElizaveta A Bonch-Osmolovskaya

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