Thermoneutrality-Induced Macrophage Accumulation in Brown Adipose Tissue Does Not Impair the Tissue's Competence for Cold-Induced Thermogenic Recruitment.

Frontiers in Endocrinology
Alexander W FischerNatasa Petrovic

Abstract

Brown adipose tissue from mice living under conditions approaching human thermal and nutritional conditions (prolonged exposure to thermoneutral temperature and to an energy-rich (high-fat, high-sugar) diet) - referred to as "physiologically humanized" mice, displays morphological and molecular characteristics significantly different from those observed in young, chow-fed mice maintained at room temperature - referred to as "standard" mice. Here, we further examined brown fat from physiologically humanized and standard mice, as well as from mice exposed to thermoneutrality for a long time but not to an energy-rich diet - referred to here as "long-term thermoneutral" mice. Global transcriptome analysis of brown fat revealed that genes that were the most upregulated in brown fat of thermoneutral mice (both physiologically humanized and long-term thermoneutral) were those related to inflammatory processes, including genes expressed selectively in macrophages. Cellular and molecular analyses confirmed that brown fat from thermoneutral mice was heavily infiltrated by macrophages, predominantly organized into crown-like structures. However, despite this, the brown fat of thermoneutral mice retained full competence to attain the great...Continue Reading

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Citations

Dec 8, 2020·American Journal of Physiology. Endocrinology and Metabolism·Nathalie BouletJan Nedergaard
Feb 7, 2021·International Journal of Molecular Sciences·Katarzyna Maliszewska, Adam Kretowski

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Methods Mentioned

BETA
PCR
RNA-Seq
PCA
FACS
confocal microscopy

Software Mentioned

GraphPad Prism
Excel
ClustVis
MATLAB

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