Thermophilic Talaromyces emersonii Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications

ACS Omega
Hisanori IwasaHitoshi Muguruma

Abstract

Flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (GDH) was identified and cloned from thermophilic filamentous fungi Talaromyces emersonii using the homology cloning method. A direct electron transfer bioanode composed of T. emersonii FAD-GDH and a single-walled carbon nanotube was produced. Enzymes from thermophilic microorganisms generally have low activity at ambient temperature; however, the T. emersonii FAD-GDH bioanode exhibits a large anodic current due to the enzymatic reaction (1 mA cm-2) at ambient temperature. Furthermore, the T. emersonii FAD-GDH bioanode worked at 70 °C for 12 h. This is the first report of a bioanode with a glucose-catalyzing enzyme from a thermophilic microorganism that has potential for biosensor and biofuel cell applications. In addition, we demonstrate how the glycoforms of T. emersonii FAD-GDHs expressed by various hosts influence the electrochemical properties of the bioanode.

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Citations

Jul 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazuya IshidaTakahide Kishimoto
Feb 6, 2021·Scientific Reports·Rosanna PuopoloGabriella Fiorentino
Jun 28, 2019·Chemical Reviews·Xinxin XiaoAihua Liu

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Methods Mentioned

BETA
biosensors
biosensor
electrophoresis
atomic force microscopy
AFM

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